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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bonde, Mads T. Pedersen, Margit Klausen, Michael S. Jensen, Sheila I. Wulff, Tune Harrison, Scott Nielsen, Alex T. Herrgård, Markus J. Sommer, Morten O. A. |
| Description | Country affiliation: Denmark Author Affiliation: Bonde MT ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Pedersen M ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Klausen MS ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Jensen SI ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Wulff T ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Harrison S ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Nielsen AT ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Herrgård MJ ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.); Sommer MO ( Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.) |
| Abstract | We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| Issue Number | 3 |
| Volume Number | 13 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2016-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Escherichia Coli Proteins Genetics Escherichia Coli Peptide Chain Initiation, Translational Protein Engineering Methods Rna, Bacterial Software Algorithms Cloning, Molecular Promoter Regions, Genetic Rna, Messenger Rna, Ribosomal, 16s Sequence Homology, Nucleic Acid Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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