NDLI: Glycogen storage and degradation during in vitro growth and differentiation of Giardia intestinalis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pradhan, Prajakta Lundgren, Scott W. Wilson, Wayne A. Brittingham, Andrew
Description	Country affiliation: United States Author Affiliation: Pradhan P ( Department of Microbiology and Immunology, Des Moines University, Des Moines, Iowa 50312, USA.)
Abstract	Giardia intestinalis is the causative agent of human giardiasis, a common diarrheal illness worldwide. Despite its global distribution and prevalence, many questions regarding its basic biology and metabolism remain unanswered. In this study, we examine the accumulation and degradation of glycogen, an important source of stored carbon and energy, during the in vitro growth and differentiation of G. intestinalis . We report that, as G. intestinalis progresses through its growth cycle, cultures of trophozoites accumulate glycogen during the lag and early logarithmic phases of growth and then utilize this compound during their remaining logarithmic growth. As cultures enter the stationary phase of growth, they re-accumulate glycogen stores. The activity of glycogen phosphorylase, an enzyme involved in glycogen metabolism, also varied throughout in vitro trophozoite growth. During the in vitro induction of trophozoite differentiation into water-resistant cyst forms, the cultures initially accumulated stores of glycogen which diminished throughout transition to the cyst form. This observation is suggestive of a role for glycogen in the differentiation process. These studies represent the first thorough analysis of changes in glycogen content and glycogen phosphorylase activity during G. intestinalis growth and differentiation.
File Format	HTM / HTML
ISSN	00223395
Issue Number	2
Volume Number	98
e-ISSN	19372345
Journal	Journal of Parasitology
Language	English
Publisher	American Society of Parasitologists
Publisher Date	2012-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Parasitology Giardia Lamblia Growth & Development Metabolism Glycogen Phosphorylase Glycogen Culture Media Diarrhea Parasitology Enzymology Giardiasis Humans Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Parasitology Ecology, Evolution, Behavior and Systematics

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