NDLI: Characterization of Toxoplasma gondii 5' UTR with encyclopedic TSS information.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yamagishi, J. Watanabe, J. Goo, Y. K. Masatani, T. Suzuki, Y. Xuan, X.
Description	Author Affiliation: Yamagishi J ( National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan 080-8555.)
Abstract	The 5' UTR is widely involved in gene expression via post-transcriptional regulation. However, a detailed profile of the 5' UTR for Toxoplasma gondii has not yet been demonstrated. To investigate the issue, we compared the predicted open reading frames (ORFs) and transcription start sites (TSSs) of T. gondii obtained by TSS-seq, a method that enables analysis of encyclopedic TSSs with next-generation sequencers. As a result, it was demonstrated that the mode length of the 5' UTR is between 120 and 140 nucleotides (nts) when a subset of genes with predicted signal peptides was examined. However, when genes without the signal peptide were examined, the length was extended to approximately 600 nts. Because additional information on the predicted signal peptide generates increased reliability to the 5' end estimation of each ORF, we believe that the former value was more reliable as a representative of the 5' UTR length of T. gondii. The discrepancy suggests that current predictions of the 5' end of the ORF were less accurate and considerably more discordant with the natural status. The 5' untranslated region (5' UTR) is defined as that between the 5' end of the transcripts and just in front of a start codon of an ORF. Therefore, the 5' UTR does not contain any information for a protein sequence; however, it is involved in the control of protein expression via the modulation of translational efficiency (Kozak, 1991b; Hughes, 2006).
File Format	HTM / HTML
ISSN	00223395
Issue Number	2
Volume Number	98
e-ISSN	19372345
Journal	Journal of Parasitology
Language	English
Publisher	American Society of Parasitologists
Publisher Date	2012-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Parasitology 5' Untranslated Regions Physiology Toxoplasma Genetics Transcription Initiation Site Expressed Sequence Tags Models, Genetic Open Reading Frames Protein Sorting Signals Protozoan Proteins Chemistry Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Parasitology Ecology, Evolution, Behavior and Systematics

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