NDLI: Influence of potent antioxidant leguminosae family plant extracts on growth and antioxidant defense system of Hep2 cancer cell line.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Spanou, Chrysoula Stagos, Dimitrios Aligiannis, Nektarios Kouretas, Demetrios
Description	Country affiliation: Greece Author Affiliation: Spanou C ( Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.)
Abstract	Legumes are considered to be a very good source of polyphenolic compounds that may act as chemopreventive agents, especially by their antioxidant properties. However, many of the chemopreventive properties may depend on the concentrations of the phytochemical compounds because potent antioxidant polyphenolic compounds may have pro-oxidant properties and negatively affect cell growth and viability. Thus, the aim of the present study was to assess the possible effect of two potent antioxidant Greek Leguminosae family plant extracts on the growth of a specific cancer cell line and its antioxidant defense cell system. Aqueous extracts of aerial parts of Lathyrus laxiflorus and Phaseolus vulgaris plants were initially examined for their cytotoxicity on the Hep2 cancer cell line at concentrations that possess potent antioxidant properties (100, 400, and 800 microg/mL). After a 24-hour incubation with the extracts, only L. laxiflorus plant extract exhibited the ability to inhibit the cell growth at 400 and 800 microg/mL by 57% and 74%, respectively, whereas P. vulgaris extract had no effect on cell growth at any of the tested concentrations. Noncytotoxic concentrations, 100 microg/mL L. laxiflorus and 800 microg/mL P. vulgaris extract, were used for 2-, 12-, and 24-hour incubation of the cells. The influence of the extracts on the antioxidant defense system of the cells was assessed by measuring the total antioxidant capacity (TAC) of the cells, the catalase (CAT) activity, and the concentrations of reduced glutathione, the oxidized form of glutathione, and thiobarbituric-reactive substances (TBARS) in all times of incubation with the cells. From the results obtained, it seems that only L. laxiflorus extract induces oxidative stress in the cells by reducing TAC and CAT activity and by inducing TBARS, especially with 2 and 12 hours of incubation. P. vulgaris extract reduced only TAC at 2 hours of incubation, indicating also a mild induction of oxidative stress. These results imply that potent antioxidant extracts, beyond a critical concentration, may induce oxidative stress even with no apparent cytotoxicity in cells.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	1
Volume Number	13
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Antineoplastic Agents, Phytogenic Pharmacology Antioxidants Metabolism Fabaceae Liver Neoplasms Drug Therapy Oxidative Stress Drug Effects Plant Extracts Therapeutic Use Catalase Cell Proliferation Glutathione Hep G2 Cells Humans Lathyrus Phaseolus Phytotherapy Thiobarbituric Acid Reactive Substances Journal Article
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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