NDLI: Antiproliferative and antitumorigenic activity of Toona sinensis leaf extracts in lung adenocarcinoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Chih-Jen Huang, Yu-Jung Wang, Cheng-Yuan Wang, Chuan-Sheng Wang, Pei-Hui Hung, Jen-Yu Wang, Tung-Heng Hsu, Hseng-Kuang Huang, Hurng-Wern Kumar, S. P. Anand Huang, Ming-Shyan Weng, Ching-Feng
Description	Country affiliation: Taiwan Author Affiliation: Yang CJ ( Department of Internal Medicine, Kaohsiung Medical University Hospital, Department of Internal Medicine, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.)
Abstract	Toona sinensis is a traditional Chinese herb, and the extracts of T. sinensis leaf possess a variety of biological functions. This study attempted to test the antiproliferative effect of TSL-1 (a bioactive fraction of T. sinensis) in H441 cells (lung adenocarcinoma). The data showed that the antiproliferative effect of TSL-1 on H441 cells is prominent using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. TSL-1-induced apoptosis was confirmed by cell morphology, sub-G(1) peak accumulation, cleavage of poly(ADP)-ribose polymerase, and propidium iodide-annexin V double staining. Furthermore, decreased Bcl-2 accompanied by increased Bax (in western blotting) was found with TSL-1 treatment of H441 cells. TSL-1 treatment-induced G(1) arrest was concurrent with the down-regulation of protein levels of cyclin D1 and cyclin-dependent kinase 4 in H441 cells. Peroral and intraperitoneal administrations of TSL-1 were performed to evaluate the therapeutic efficacy, and peroral administration of TSL-1 was also used to elucidate the therapeutic efficacy in the H441 cell xenograft model in vivo. The data revealed that TSL-1 treatment inhibited H441 tumor growth in both therapeutic and preventive experiments. Taken together, these results demonstrate that TSL-1 possesses the capability of preventing and alleviating lung cancer proliferation in vitro and in vivo with proven nephrological and hepatic safety and has the potential to be developed as an anti-lung cancer drug.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	1
Volume Number	13
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Adenocarcinoma Drug Therapy Antineoplastic Agents, Phytogenic Therapeutic Use Apoptosis Drug Effects Cedrela Cell Proliferation Lung Neoplasms Plant Extracts Metabolism Animals Administration & Dosage Pharmacology Cell Cycle Cell Line, Tumor Cyclin D1 Cyclin-dependent Kinase 4 Down-regulation Humans Mice Mice, Inbred Balb C Mice, Nude Phytotherapy Plant Leaves Proto-oncogene Proteins C-bcl-2 Xenograft Model Antitumor Assays Bcl-2-associated X Protein Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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