NDLI: Regenerative capacity of the adult pituitary: multiple mechanisms of lactotrope restoration after transgenic ablation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fu, Qiuli Vankelecom, Hugo
Description	Country affiliation: Belgium Author Affiliation: Fu Q ( Laboratory of Tissue Plasticity, Research Unit of Embryo and Stem Cells, Department of Development and Regeneration, University of Leuven (KU Leuven), Leuven, Belgium.)
Abstract	In a recent study, we showed that the adult pituitary gland is capable of regenerating transgenically ablated growth hormone-producing (GH(+)) somatotropes. Here, we investigated whether the gland's regenerative capacity is more general and also applies to the other major hormonal cell type, the prolactin-producing (PRL(+)) lactotropes. We set up the transgenic PRLCre/inducible diphtheria toxin receptor (iDTR) mouse model, in which the PRL promoter drives expression of Cre that induces DTR in lactotropes. Injection of female mice with DT for different periods causes a gradual ablation of PRL(+) cells, reaching a maximum of 70% after 10-day DT treatment. During the following weeks, lactotropes progressively reappear achieving a 60% restoration after 6 weeks. The Sox2(+) stem/progenitor cell compartment displays a prompt reaction to the DT-triggered cell ablation injury, including expansion of the marginal-zone niche and coexpression of PRL, the latter only very rarely observed in control pituitary. Throughout the regeneration period (2-6 weeks), Sox2(+) as well as double Sox2(+)/PRL(+) cells continue to be more abundant than in control pituitary. In addition to this stem cell reaction, surviving or newborn lactotropes increase their proliferative activity, and bihormonal PRL(+)/GH(+) cells become detectable suggesting somatotrope-to-lactotrope transdifferentiation. In conclusion, the adult pituitary gland is capable of restoring lactotrope cells after destruction, further confirming its regenerative competence. Repair of lactotropes appears to be driven by a combination of mechanisms, including recruitment from stem cells, proliferation of lactotropes, and transdifferentiation of somatotropes.
File Format	HTM / HTML
ISSN	15473287
Issue Number	18
Volume Number	21
e-ISSN	15578534
Journal	Stem Cells and Development
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2012-12-10
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Cell Biology Discipline Hematology Lactotrophs Physiology Pituitary Gland Regeneration Somatotrophs Animals Cell Proliferation Cell Transdifferentiation Diphtheria Toxin Pharmacology Female Growth Hormone Biosynthesis Heparin-binding Egf-like Growth Factor Intercellular Signaling Peptides And Proteins Genetics Mice Mice, Inbred C57bl Mice, Transgenic Cytology Metabolism Prolactin Promoter Regions, Genetic Soxb1 Transcription Factors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Hematology

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