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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Hong-Chao Liu, Xin-Bin Huang, Shu Bi, Xiao-Yun Wang, Heng-Xiang Xie, Li-Xian Wang, Yong-Qi Cao, Xiao-Fang Lv, Jun Xiao, Feng-Jun Yang, Yang Guo, Zi-Kuan |
| Description | Country affiliation: China Author Affiliation: Zhang HC ( Department of Cardiology Surgery, General Hospital of Air Force, Beijing, China.) |
| Abstract | Although mesenchymal stem cells (MSCs) have been increasingly trialed to treat a variety of diseases, the underlying mechanisms remain still elusive. In this study, human umbilical cord (UC)-derived MSCs were stimulated by hypoxia, and the membrane microvesicles (MVs) in the supernatants were collected by ultracentrifugation, observed under an electron microscope, and the origin was identified with the flow cytometric technique. The results showed that upon hypoxic stimulus, MSCs released a large quantity of MVs of ~100 nm in diameter. The MVs were phenotypically similar to the parent MSCs, except that the majority of them were negative for the receptor of platelet-derived growth factor. DiI-labeling assay revealed that MSC-MVs could be internalized into human UC endothelial cells (UC-ECs) within 8 h after they were added into the culture medium. Carboxyfluorescein succinimidyl ester-labeling technique and MTT test showed that MSC-MVs promoted the proliferation of UC-ECs in a dose-dependent manner. Further, MVs could enhance in vitro capillary network formation of UC-ECs in a Matrigel matrix. In a rat hindlimb ischemia model, both MSCs and MSC-MVs were shown to improve significantly the blood flow recovery compared with the control medium (P<0.0001), as assessed by laser Doppler imaging analysis. These data indicate that MV releasing is one of the major mechanisms underlying the effectiveness of MSC therapy by promoting angiogenesis. |
| File Format | HTM / HTML |
| ISSN | 15473287 |
| e-ISSN | 15578534 |
| DOI | 10.1089/scd.2012.0095 |
| Journal | Stem Cells and Development |
| Issue Number | 18 |
| Volume Number | 21 |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2012-12-10 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Cell Biology Discipline Hematology Cell Membrane Structures Physiology Hindlimb Blood Supply Human Umbilical Vein Endothelial Cells Metabolism Mesenchymal Stromal Cells Cytology Neovascularization, Physiologic Animals Cell Differentiation Cell Hypoxia Cell Proliferation Cells, Cultured Flow Cytometry Fluoresceins Ischemia Receptors, Platelet-derived Growth Factor Succinimides Umbilical Cord Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Hematology |
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