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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Martínez-González, Itziar Moreno, Rafael Petriz, Jordi Gratacós, Eduard Aran, Josep M. |
| Description | Country affiliation: Spain Author Affiliation: Martínez-González I ( Human Molecular Genetics Group, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Hospital Duran i Reynals, L'Hospitalet de Llobregat, Barcelona, Spain.) |
| Abstract | Due to their favorable intrinsic features, including engraftment, differentiation, and immunomodulatory potential, adult mesenchymal stem cells (MSCs) have been proposed for therapeutic in utero intervention. Further improvement of such attributes for particular diseases might merely be achieved by ex vivo MSC genetic engineering previous to transplantation. Here, we evaluated for the first time the feasibility, biodistribution, long-term engraftment, and transgenic enhanced green fluorescent protein (EGFP) expression of genetically engineered human adipose tissue-derived MSCs (EGFP(+)-ASCs) after intra-amniotic xenotransplantation at E17 of gestation into our validated pregnant rabbit model. Overall, the procedure was safe (86.4% survival rate; absence of anatomical defects). Stable, low-level engraftment of EGFP(+)-ASCs was confirmed by assessing the presence of the pWT-EGFP lentiviral provirus in the young transplanted rabbit tissues. Accordingly, similar frequencies of provirus-positive animals were found at both 8 weeks (60%) and 16 weeks (66.7%) after in utero intervention. The presence of EGFP(+)-ASCs was more frequent in respiratory epithelia (lung and trachea), according to the route of administration. However, we were unable to detect EGFP expression, neither by real-time polymerase chain reaction nor by immunohistochemistry, in the provirus-positive tissues, suggesting EGFP transgene silencing mediated by epigenetic events. Moreover, we noticed lack of both host cellular immune responses against xenogeneic ASCs and humoral immune responses against transgenic EGFP. Therefore, the fetal microchimerism achieved by the EGFP(+)-ASCs in the young rabbit hosts indicates induction of donor-specific tolerance after fetal rabbit xenotransplantation, which should boost postnatal transplantation for the early treatment/prevention of many devastating congenital disorders. |
| File Format | HTM / HTML |
| ISSN | 15473287 |
| e-ISSN | 15578534 |
| DOI | 10.1089/scd.2012.0032 |
| Journal | Stem Cells and Development |
| Issue Number | 18 |
| Volume Number | 21 |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2012-12-10 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Cell Biology Discipline Hematology Adipose Tissue Cytology Adult Stem Cells Transplantation Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells Animals Animals, Genetically Modified Cell Differentiation Fetus Genetic Engineering Genetic Vectors Green Fluorescent Proteins Biosynthesis Genetics Lymphocyte Culture Test, Mixed Rabbits Transplantation, Heterologous Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Hematology |
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