NDLI: Cell growth characteristics, differentiation frequency, and immunophenotype of adult ear mesenchymal stem cells.

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Cell growth characteristics, differentiation frequency, and immunophenotype of adult ear mesenchymal stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Staszkiewicz, Jaroslaw Frazier, Trivia P. Rowan, Brian G. Bunnell, Bruce A. Chiu, Ernest S. Gimble, Jeffrey M. Gawronska-Kozak, Barbara
Description	Country affiliation: Poland Author Affiliation: Staszkiewicz J ( Regenerative Biology Laboratory, Louisiana State University System, Baton Rouge, Louisiana. , Department of Animal Physiology, University of Warmia and Mazury, Olsztyn, Poland.)
Abstract	Ear mesenchymal stem cells (EMSCs) represent a readily accessible population of stem-like cells that are adherent, clonogenic, and have the ability to self-renew. Previously, we have demonstrated that they can be induced to differentiate into adipocyte, osteocyte, chondrocyte, and myocyte lineages. The purpose of the current study was to characterize the growth kinetics of the cells and to determine their ability to form colonies of fibroblasts, adipocytes, osteocytes, and chondrocytes. In addition, the immunophenotypes of freshly isolated and culture-expanded cells were evaluated. From 1 g of tissue, we were able to isolate an average of 7.8 x 10(6) cells exhibiting a cell cycle length of approximately 2-3 days. Colony-forming unit (CFU) assays indicated high proliferation potential, and confirmed previously observed multipotentiality of the cells. Fluorescence-activated cell sorting (FACS) showed that EMSCs were negative for hematopoietic markers (CD4, CD45), proving that they did not derive from circulating hematopoietic cells. The FACS analyses also showed high expression of stem cell antigen-1 (Sca-1) with only a minor population of cells expressing CD117, thus identifying Sca-1 as the more robust stem cell biomarker. Additionally, flow cytometry data revealed that the expression patterns of hematopoietic, stromal, and stem cell markers were maintained in the passaged EMSCs, consistent with the persistence of an undifferentiated state. This study indicates that EMSCs provide an alternative model for in vitro analyses of adult mesenchymal stem cells (MSCs). Further studies will be necessary to determine their utility for tissue engineering and regenerative medical applications.
File Format	HTM / HTML
ISSN	15473287
e-ISSN	15578534
DOI	10.1089/scd.2009.0051
Journal	Stem Cells and Development
Issue Number	1
Volume Number	19
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Discipline Hematology Cell Differentiation Physiology Cell Proliferation Ear Immunophenotyping Mesenchymal Stromal Cells Adult Stem Cells Metabolism Animals Biological Markers Cell Movement Colony-forming Units Assay Efficiency Mice Mice, Inbred C57bl Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Hematology

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