NDLI: Collection protocol for human pancreas.

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Collection protocol for human pancreas.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Campbell-Thompson, Martha L. Montgomery, Emily L. Foss, Robin M. Kolheffer, Kerwin M. Phipps, Gerald Schneider, Lynda Atkinson, Mark A.
Description	Country affiliation: United States Author Affiliation: Campbell-Thompson ML ( Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, USA. mct@ufl.edu)
Abstract	This dissection and sampling procedure was developed for the Network for Pancreatic Organ Donors with Diabetes (nPOD) program to standardize preparation of pancreas recovered from cadaveric organ donors. The pancreas is divided into 3 main regions (head, body, tail) followed by serial transverse sections throughout the medial to lateral axis. Alternating sections are used for fixed paraffin and fresh frozen blocks and remnant samples are minced for snap frozen sample preparations, either with or without RNAse inhibitors, for DNA, RNA, or protein isolation. The overall goal of the pancreas dissection procedure is to sample the entire pancreas while maintaining anatomical orientation. Endocrine cell heterogeneity in terms of islet composition, size, and numbers is reported for human islets compared to rodent islets. The majority of human islets from the pancreas head, body and tail regions are composed of insulin-containing ß-cells followed by lower proportions of glucagon-containing -cells and somatostatin-containing Î´-cells. Pancreatic polypeptide-containing PP cells and ghrelin-containing epsilon cells are also present but in small numbers. In contrast, the uncinate region contains islets that are primarily composed of pancreatic polypeptide-containing PP cells. These regional islet variations arise from developmental differences. The pancreas develops from the ventral and dorsal pancreatic buds in the foregut and after rotation of the stomach and duodenum, the ventral lobe moves and fuses with the dorsal. The ventral lobe forms the posterior portion of the head including the uncinate process while the dorsal lobe gives rise to the rest of the organ. Regional pancreatic variation is also reported with the tail region having higher islet density compared to other regions and the dorsal lobe-derived components undergoing selective atrophy in type 1 diabetes. Additional organs and tissues are often recovered from the organ donors and include pancreatic lymph nodes, spleen and non-pancreatic lymph nodes. These samples are recovered with similar formats as for the pancreas with the addition of isolation of cryopreserved cells. When the proximal duodenum is included with the pancreas, duodenal mucosa may be collected for paraffin and frozen blocks and minced snap frozen preparations.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/4039
Journal	Journal of Visualized Experiments
Issue Number	63
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-05-23
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Pancreas Anatomy & Histology Surgery Tissue And Organ Harvesting Dissection Organ Preservation Cytology Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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