NDLI: Engineering a bilayered hydrogel to control ASC differentiation.

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Engineering a bilayered hydrogel to control ASC differentiation.

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Engineering a bilayered hydrogel to control ASC differentiation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Natesan, Shanmugasundaram Zamora, David O. Suggs, Laura J. Christy, Robert J.
Description	Country affiliation: United States Author Affiliation: Natesan S ( Department of Extremity Trauma Research and Regenerative Medicine, United States Army Institute of Surgical Research, USA.)
Abstract	Natural polymers over the years have gained more importance because of their host biocompatibility and ability to interact with cells in vitro and in vivo. An area of research that holds promise in regenerative medicine is the combinatorial use of novel biomaterials and stem cells. A fundamental strategy in the field of tissue engineering is the use of three-dimensional scaffold (e.g., decellularized extracellular matrix, hydrogels, micro/nano particles) for directing cell function. This technology has evolved from the discovery that cells need a substrate upon which they can adhere, proliferate, and express their differentiated cellular phenotype and function. More recently, it has also been determined that cells not only use these substrates for adherence, but also interact and take cues from the matrix substrate (e.g., extracellular matrix, ECM). Therefore, the cells and scaffolds have a reciprocal connection that serves to control tissue development, organization, and ultimate function. Adipose-derived stem cells (ASCs) are mesenchymal, non-hematopoetic stem cells present in adipose tissue that can exhibit multi-lineage differentiation and serve as a readily available source of cells (i.e. pre-vascular endothelia and pericytes). Our hypothesis is that adipose-derived stem cells can be directed toward differing phenotypes simultaneously by simply co-culturing them in bilayered matrices. Our laboratory is focused on dermal wound healing. To this end, we created a single composite matrix from the natural biomaterials, fibrin, collagen, and chitosan that can mimic the characteristics and functions of a dermal-specific wound healing ECM environment.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/3953
Journal	Journal of Visualized Experiments
Issue Number	63
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-05-25
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Adipose Tissue Cytology Biopolymers Chemistry Hydrogels Mesenchymal Stromal Cells Animals Biomimetic Materials Pharmacology Cell Differentiation Drug Effects Physiology Chitosan Coculture Techniques Collagen Extracellular Matrix Fibrin Tissue Scaffolds Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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