NDLI: Biocompatibility of individually designed scaffolds with human periosteum for use in tissue engineering.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Becker, Stephan T. Douglas, Timothy Acil, Yahya Seitz, Hermann Sivananthan, Sureshan Wiltfang, Jörg Warnke, Patrick H.
Description	Country affiliation: Germany Author Affiliation: Becker ST ( Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University of Kiel, Kiel, Germany. becker@mkg.uni-kiel.de)
Abstract	UNLABELLED: The aim of this study was to evaluate and compare the biocompatibility of computer-assisted designed (CAD) synthetic hydroxyapatite (HA) and tricalciumphosphate (TCP) blocks and natural bovine hydroxyapatite blocks for augmentations and endocultivation by supporting and promoting the proliferation of human periosteal cells. Human periosteum cells were cultured using an osteogenic medium consisting of Dulbecco's modified Eagle medium supplemented with fetal calf serum, Penicillin, Streptomycin and ascorbic acid at 37 degrees C with 5% CO(2). Three scaffolds were tested: 3D-printed HA, 3D-printed TCP and bovine HA. Cell vitality was assessed by Fluorescein Diacetate (FDA) and Propidium Iodide (PI) staining, biocompatibility with LDH, MTT, WST and BrdU tests, and scanning electron microscopy. Data were analyzed with ANOVAs. RESULTS: After 24 h all samples showed viable periosteal cells, mixed with some dead cells for the bovine HA group and very few dead cells for the printed HA and TCP groups. The biocompatibility tests revealed that proliferation on all scaffolds after treatment with eluate was sometimes even higher than controls. Scanning electron microscopy showed that periosteal cells formed layers covering the surfaces of all scaffolds 7 days after seeding. CONCLUSION: It can be concluded from our data that the tested materials are biocompatible for periosteal cells and thus can be used as scaffolds to augment bone using tissue engineering methods.
File Format	HTM / HTML
ISSN	09574530
Issue Number	4
Volume Number	21
e-ISSN	15734838
Journal	Journal of Materials Science: Materials in Medicine
Language	English
Publisher	Springer
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biomedical Engineering Materials Testing Periosteum Physiology Tissue Engineering Tissue Scaffolds Animals Cattle Cell Adhesion Drug Effects Cell Culture Techniques Cell Proliferation Cell Survival Cells, Cultured Durapatite Chemistry Pharmacology Humans Models, Biological Osteogenesis Cytology Prosthesis Design Methods Adverse Effects Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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