NDLI: Sensing of protein adsorption with a porous bulk composite comprising silver nanoparticles deposited on hydroxyapatite.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ohtsuki, Chikara Ichikawa, Yuji Shibata, Hiroyuki Kawachi, Giichiro Torimoto, Tsukasa Ogata, Shin-ichi
Description	Country affiliation: Japan Author Affiliation: Ohtsuki C ( Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan. ohtsuki@apchem.nagoya-u.ac.jp)
Abstract	Porous bulk composites were produced by depositing silver nanoparticles of diameter 11.0 +/- 3.2 nm on hydroxyapatite of micrometer sizes. Adsorption of bovine serum albumin (BSA) and lysozyme (LSZ) on the composite material was observed in 2 and 10 mol m(-3) phosphate buffer solutions. More BSA than LSZ was adsorbed in 2 mol m(-3) phosphate buffer and this was attributed to a larger a-face surface area present in the plate- and rod-shaped hydroxyapatite compared with the c-face surface area. Peak shifts in localized surface plasmon resonance (LSPR) spectra were clearly related to adsorbed amounts of BSA and LSZ after exposure of the porous bulk composites to protein solutions. The sensing capability of the porous bulk composite results from changes in the dielectric constant of the surface fluid surrounding the silver nanoparticles. Adsorption/desorption cycles of BSA were applied to the porous bulk composite, confirming the reversibility of the sensing capability.
File Format	HTM / HTML
ISSN	09574530
Issue Number	4
Volume Number	21
e-ISSN	15734838
Journal	Journal of Materials Science: Materials in Medicine
Language	English
Publisher	Springer
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biomedical Engineering Biosensing Techniques Instrumentation Durapatite Chemistry Metal Nanoparticles Nanocomposites Proteins Pharmacokinetics Silver Adsorption Animals Cattle Coated Materials, Biocompatible Models, Biological Muramidase Metabolism Particle Size Porosity Protein Binding Serum Albumin, Bovine Surface Plasmon Resonance Surface Properties Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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