NDLI: High-throughput approaches to measuring cell death.

Please wait, while we are loading the content...

Year: 2017 Volume: 2017

Year: 2016 Volume: 2016

Year: 2015 Volume: 2015

Year: 2014 Volume: 2014

Year: 2014 Volume: 2014 Issue: 12

Year: 2014 Volume: 2014 Issue: 10

Year: 2014 Volume: 2014 Issue: 8

Year: 2014 Volume: 2014 Issue: 7

Year: 2014 Volume: 2014 Issue: 6

Measuring Endogenous $I_{CRAC}$ and ORAI Currents with the Patch-Clamp Technique

The Minimal Requirements to Use Calcium Imaging to Analyze $I_{CRAC}$

Manual hematoxylin and eosin staining of mouse tissue sections.

Manual immunohistochemistry staining of mouse tissues using the avidin-biotin complex (ABC) technique.

Purification of pericytes from rodent optic nerve by immunopanning.

A high-throughput, multiplex cell death assay using an RNAi screening approach.

3'-End labeling of RNA with yeast Poly(A) polymerase and 3'-deoxyadenosine 5'-[ -32P]triphosphate.

RNase footprinting to map sites of RNA-protein interactions.

Reverse footprinting to map sites of RNA-protein interactions.

High-speed two-photon calcium imaging of neuronal population activity using acousto-optic deflectors.

Monitoring membrane potential with second-harmonic generation.

Patch-Clamp Measurement of $I_{CRAC}$ and ORAI Channel Activity

Analysis of mouse model pathology: a primer for studying the anatomic pathology of genetically engineered mice.

Purification and culture of central nervous system pericytes.

High-throughput approaches to measuring cell death.

Nitrophenyl-based caged neurotransmitters.

Year: 2014 Volume: 2014 Issue: 4

Year: 2014 Volume: 2014 Issue: 3

Year: 2014 Volume: 2014 Issue: 2

Year: 2014 Volume: 2014 Issue: 1

Year: 2013 Volume: 2013

Year: 2012 Volume: 2012

Year: 2011 Volume: 2011

Year: 2010 Volume: 2010

Year: 2009 Volume: 2009

Year: 2008 Volume: 2008

Year: 2007 Volume: 2007

High-throughput approaches to measuring cell death.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Saunders, Darren N. Falkenberg, Katrina J. Simpson, Kaylene J.
Description	Country affiliation: Australia Author Affiliation: Saunders DN ( Cancer Research Program, The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010, Australia St. Vincent's Clinical School, University of New South Wales Medicine, Sydney, New South Wales 2000, Australia.); Falkenberg KJ ( Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia Department of Pathology, The University of Melbourne, Parkville, Victoria 3052, Australia.); Simpson KJ ( Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia Department of Pathology, The University of Melbourne, Parkville, Victoria 3052, Australia Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria 3052, Australia.)
Abstract	Cell death is integral to developmental and disease processes, and high-throughput screening (HTS) has been instrumental both for understanding biological mechanisms underlying cell death and for discovering novel therapeutic agents targeting these pathways. The various cell death modalities and their distinctive morphological and biochemical features have led to the development of a staggering variety of assays to measure these features, many of which have been adapted to HTS format. Although not all cell death assays are readily amenable to a high-throughput format, the potential power of HTS assays and increasing accessibility to associated technology make it likely that new approaches will continue to emerge. In particular, many recent studies in this field have used multiplex assays and high-content imaging to measure several features concurrently. Here, we discuss a broad array of considerations for designing HTS cell death assays, including some common challenges and pitfalls. We aim to provide a framework for deciding the most appropriate biological readouts, assay strategy and mode, workflow, controls, validation, and bioinformatics.
File Format	HTM / HTML
ISSN	19403402
Issue Number	6
Volume Number	2014
e-ISSN	15596095
Journal	Cold Spring Harbor Protocols
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2014-06-02
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Cell Death Cytological Techniques Methods High-throughput Screening Assays Cell Line Cell Survival Fluorescent Dyes Analysis Humans Staining And Labeling Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A high-throughput, multiplex cell death assay using an RNAi screening approach.

Efficient production of a gene mutant cell line through integrating TALENs and high-throughput cell cloning.

Labeling membranes with fluorescent phosphatidylethanolamine.

Coating particles with dextran-conjugated fluorescent dyes or other hydrophilic compounds.

Measuring $Ca^{2+}$ Sparks in Cardiac Myocytes

Coating particles with carbocyanine dyes.

Fluorescent speckle microscopy.

Labeling membranes with fluorescent cholesterols.

Fluorescent labeling of membrane lipid rafts.

High-throughput approaches to measuring cell death.

Similar Documents

A high-throughput, multiplex cell death assay using an RNAi screening approach.

Efficient production of a gene mutant cell line through integrating TALENs and high-throughput cell cloning.

Labeling membranes with fluorescent phosphatidylethanolamine.

Coating particles with dextran-conjugated fluorescent dyes or other hydrophilic compounds.

Measuring $Ca^{2+}$ Sparks in Cardiac Myocytes

Coating particles with carbocyanine dyes.

Fluorescent speckle microscopy.

Labeling membranes with fluorescent cholesterols.

Fluorescent labeling of membrane lipid rafts.

High-throughput approaches to measuring cell death.