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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cardiff, Robert D. Miller, Claramae H. Munn, Robert J. |
| Description | Author Affiliation: Cardiff RD ( Center for Comparative Medicine and Center for Genomic Pathology, University of California, Davis, Davis, California 95616.); Miller CH ( Center for Comparative Medicine and Center for Genomic Pathology, University of California, Davis, Davis, California 95616.); Munn RJ ( Center for Comparative Medicine and Center for Genomic Pathology, University of California, Davis, Davis, California 95616.) |
| Abstract | There are many variations on the immunohistochemistry (IHC) procedure, but all are based on attachment of a primary antibody to a unique epitope on or within the cell. This step is followed by incubation of the cell/primary antibody complex with another, secondary antibody that recognizes the species in which the primary antibody was produced. The secondary antibody has an indicator molecule attached to it. The indicator produces a colored reaction product at the site of original epitope, allowing visualization. This basic two-antibody 'sandwich' procedure has many modifications that include other layers of antibodies and numerous indicators, but all variations depend upon the unique ability of antibodies to recognize specific epitopes or antigenic determinants. The procedure described here is called the ABC (avidin-biotin complex) technique. The method utilizes the high avidity of biotin for avidin, which allows formation of a strong bond. The reagents described in this technique produce a gold/brown reaction product that identifies the epitope of interest. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 6 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2014 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2014-06-02 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Avidin Metabolism Biotin Immunohistochemistry Methods Staining And Labeling Animals Mice Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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