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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Grewe, Benjamin F. Helmchen, Fritjof |
| Abstract | Two-photon calcium imaging of neuronal populations allows optical measurements of spiking activity in living animals. However, laser-scanning microscopes with galvanometric scan mirrors are too slow to capture population activity on a millisecond timescale. This protocol describes a two-photon microscope that is based on two-dimensional laser scanning with acousto-optic deflectors (AODs), enabling high-speed in vivo recording of neuronal population activity at temporal resolutions of several hundred hertz. The detailed construction plan of the AOD-based microscope is accompanied by equally detailed optimization procedures. We also introduce a novel random-access pattern scanning (RAPS) technique for high-speed in vivo measurements of neuronal population activity. AOD-based RAPS can measure calcium transients in neocortical neuronal populations, revealing spike trains with near-millisecond precision. The current limitations of the AOD-based microscope are discussed, and we provide an outlook of its future applications. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 6 |
| Volume Number | 2014 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor Protocols |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2014-06-02 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Action Potentials Calcium Metabolism Microscopy, Confocal Methods Neurons Physiology Optical Imaging Animals Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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