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| Content Provider | frontiers |
|---|---|
| Author | Yamaguchi, Akihiro Wu, Rui McNulty, Paul Mihovilovic Skanata, Mirna Gershow, Marc |
| Abstract | To understand how neural activity enccodes and coordinates behavior, it is desirable to record multi-neuronal activity in freely behaving animals. Imaging in unrestrained animals is challenging, especially for those, like larval \textit{Drosophila melanogaster}, whose brains are deformed by body motion. A previously demonstrated two-photon tracking microscope recorded from individual neurons in freely crawling \textit{Drosophila} larvae but faced limits in multi-neuronal recording. Here we demonstrate a new tracking microscope using acousto-optic deflectors (AODs) and an acoustic GRIN lens (TAG lens) to achieve axially resonant 2D random access scanning, sampling along arbitrarily located axial lines at a line rate of 70 kHz. With a tracking latency of 0.1 ms, this microscope recorded activities of various neurons in moving larval \textit{Drosophila} CNS and VNC including premotor neurons, bilateral visual interneurons, and descending command neurons. This technique can be applied to the existing two-photon microscope to allow for fast 3D tracking and scanning. |
| ISSN | 1662453X |
| DOI | 10.3389/fnins.2023.1135457 |
| Volume Number | 17 |
| Journal | Frontiers in Neuroscience |
| Language | English |
| Publisher Date | 2023-06-14 |
| Access Restriction | Open |
| Subject Keyword | Motor system Two-photon TAG lens Tracking microscopy Acousto-optic Drosophila larva Drosophila Calcium imagaing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience |
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