| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Kandhare, Amit D. Bodhankar, Subhash L. Mohan, Vishwaraman Thakurdesai, Prasad A. |
| Description |
Country affiliation: India Author Affiliation: Kandhare AD ( Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune 411 038, India.); Bodhankar SL ( Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune 411 038, India. Electronic address: sbodhindus@gmail.com.); Mohan V ( Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, Kondhwa, Pune 411 048, India.); Thakurdesai PA ( Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, Kondhwa, Pune 411 048, India.) |
| Abstract |
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic progressive multifactorial disease with limited therapeutic options. Glycosides based standardized fenugreek seed extract (SFSE-G) possesses potent anti-inflammatory and anti-oxidant property. AIM: To evaluate the efficacy of SFSE-G against bleomycin (BLM) induced pulmonary fibrosis by assessing behavioral, biochemical, molecular and ultrastructural changes in the laboratory rats. MATERIALS AND METHODS: IPF was induced in male Sprague-Dawley rats by single intratracheal BLM (6IU/kg) injection followed by SFSE-G (5, 10, 20 and 40mg/kg, p.o.) or methylprednisolone (10mg/kg, p.o.) treatment for 28day. Various parameters were analyzed in lung and bronchoalveolar lavage fluid (BALF) after 14 and 28days of the drug treatment. RESULTS: SFSE-G (20 and 40mg/kg, p.o.) administration significantly prevented the BLM induced alteration in body weight, lung index, lung function test and hematology. The altered total and differential cell count in BALF and blood was significantly prevented by SFSE-G treatment. The decreased peripheral blood oxygen content after BLM instillation was significantly increased by SFSE-G treatment. SFSE-G significantly enhanced the BALF and lung antioxidant status, through modulating the SOD, GSH, T-AOC, MDA, NO level and Nrf2, HO-1 mRNA expression. There was a significant reduction in lung 5-HT level by SFSE-G treatment. The altered mRNA expression of biomarkers of lung inflammation (TNF- , IL-1ß, IL-6 and IL-8), fibrosis (TGF-ß, collagen-1, ET-1, Muc5ac, NF-κB, VEGF, Smad-3) and apoptosis (Bax, Bcl-2 and Caspase-3) were significantly prevented by SFSE-G treatment. BLM induced histological inflammatory and fibrotic insult in the lung were reduced by SFSE-G treatment. It also ameliorated BLM induced lung ultrastructural changes as observed by transmission electron microscopic studies. However, administration of SFSE-G (5mg/kg, p.o.) failed to show any protective effect against BLM-induced PF whereas SFSE-G (10mg/kg, p.o.) showed significant amelioration in BLM-induced PF except lung function test, BALF and lung antioxidant level. CONCLUSION: SFSE-G showed anti-fibrotic efficacy executed through induction of Nrf2, which in turn may modulate anti-inflammatory molecules, inhibit fibrogenic molecules and decreased apoptosis to ameliorate BLM induced pulmonary fibrosis. |
| File Format |
HTM / HTML |
| ISSN |
00092797 |
| Volume Number |
237 |
| e-ISSN |
18727786 |
| Journal |
Chemico-Biological Interactions |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-07-25 |
| Publisher Place |
Ireland |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Biochemistry Discipline Pharmacology Bleomycin Toxicity Glycosides Pharmacology Plant Extracts Pulmonary Fibrosis Chemically Induced Seeds Chemistry Trigonella Alkaline Phosphatase Blood Animals Bronchoalveolar Lavage Fluid Interleukin-1beta Physiology L-lactate Dehydrogenase Male Mucin 5ac Nf-e2-related Factor 2 Nf-kappa B Standards Physiopathology Rats Rats, Sprague-dawley Reverse Transcriptase Polymerase Chain Reaction Embryology Tumor Necrosis Factor-alpha Bcl-2-associated X Protein Journal Article |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Toxicology |
