| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Nicolau, Lucas A. D. Carvalho, Nathalia S. Pacífico, Dvison M. Lucetti, Larisse T. Aragão, Karoline S. Véras, Leiz M. C. Souza, Marcellus H. L. P. Leite, José R. S. A. Medeiros, Jand Venes R. |
| Description |
Country affiliation: Brazil Author Affiliation: Nicolau LA ( Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.); Carvalho NS ( Post Graduation Program in Pharmacology, Federal University of Piauí, Teresina, PI, Brazil.); Pacífico DM ( Post Graduation Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, Fortaleza, CE, Brazil.); Lucetti LT ( Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.); Aragão KS ( Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.); Véras LM ( Post Graduation Program in Biotechnology, Federal University of Piauí, Parnaíba, Brazil.); Souza MH ( Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.); Leite JR ( Post Graduation Program in Biotechnology, Federal University of Piauí, Parnaíba, Brazil.); Medeiros JV ( Post Graduation Program in Pharmacology, Federal University of Piauí, Teresina, PI, Brazil) |
| Abstract |
OBJECTIVE: This study aimed to investigate the protective effect of epiisopiloturine hydrochloride (EPI), an imidazole alkaloid, on NAP-induced gastrointestinal damage in rats. METHODS: Initially, rats were pretreated with 0.5% carboxymethylcellulose (vehicle) or EPI (3, 10 and 30mg/kg, p.o. or i.p., groups 3-5, respectively) twice daily, for 2days. After 1h, NAP (80mg/kg, p.o.) was given. The control group received only vehicle (group 1) or vehicle+naproxen (group 2). Rats were euthanized on 2nd day, 4h after NAP treatment. Stomachs lesions were measured. Samples were collected for histological evaluation and glutathione (GSH), malonyldialdehyde (MDA), myeloperoxidase (MPO), and cytokines levels. Moreover, gastric mucosal blood flow (GMBF) was evaluated. RESULTS: EPI pretreatment prevented NAP-induced macro and microscopic gastric damage with a maximal effect at 10mg/kg. Histological analysis revealed that EPI decreased scores of damage caused by NAP. EPI reduced MPO (3.4±0.3U/mg of gastric tissue) and inhibited changes in MDA (70.4±8.3mg/g of gastric tissue) and GSH (246.2±26.4mg/g of gastric tissue). NAP increased TNF- levels, and this effect was reduced by EPI pretreatment. Furthermore, EPI increased GMBF by 15% compared with the control group. CONCLUSION: Our data show that EPI protects against NAP-induced gastric and intestinal damage by reducing pro-inflammatory cytokines, reducing oxidative stress, and increasing GMBF. |
| File Format |
HTM / HTML |
| ISSN |
07533322 |
| Volume Number |
87 |
| e-ISSN |
19506007 |
| Journal |
Biomedicine & Pharmacotherapy |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2017-03-01 |
| Publisher Place |
France |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Pharmacology 4-butyrolactone Analogs & Derivatives Alkaloids Therapeutic Use Gastrointestinal Diseases Prevention & Control Imidazoles Naproxen Toxicity Pilocarpus Plant Extracts Pharmacology Isolation & Purification Animals Dose-response Relationship, Drug Gastric Mucosa Drug Effects Pathology Chemically Induced Intestinal Mucosa Male Plant Leaves Protective Agents Rats Rats, Wistar Journal Article |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Pharmacology |
