NDLI: Sialic acid-dependent interactions between influenza viruses and Streptococcus suis affect the infection of porcine tracheal cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Nai-Huei Meng, Fandan Seitz, Maren Valentin-Weigand, Peter Herrler, Georg
Description	Country affiliation: Germany Author Affiliation: Wu NH ( 1âInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.); Meng F ( 1âInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.); Seitz M ( 2âInstitute of Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.); Valentin-Weigand P ( 2âInstitute of Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.); Herrler G ( 1âInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.)
Abstract	Bacterial co-infections are a major complication in influenza-virus-induced disease in both humans and animals. Either of the pathogens may induce a host response that affects the infection by the other pathogen. A unique feature in the co-infection by swine influenza viruses (SIV) and Streptococcus suis serotype 2 is the direct interaction between the two pathogens. It is mediated by the haemagglutinin of SIV that recognizes the 2,6-linked sialic acid present in the capsular polysaccharide of Streptococcus suis. In the present study, this interaction was demonstrated for SIV of both H1N1 and H3N2 subtypes as well as for human influenza viruses that recognize 2,6-linked sialic acid. Binding of SIV to Streptococcus suis resulted in co-sedimentation of virus with bacteria during low-speed centrifugation. Viruses bound to bacteria retained infectivity but induced only tiny plaques compared with control virus. Infection of porcine tracheal cells by SIV facilitated adherence of Streptococcus suis, which was evident by co-staining of bacterial and viral antigen. Sialic-acid-dependent binding of Streptococcus suis was already detectable after incubation for 30âmin. By contrast, bacterial co-infection had a negative effect on the replication of SIV as indicated by lower virus titres in the supernatant and a delay in the kinetics of virus release.
File Format	HTM / HTML
ISSN	00221317
Issue Number	9
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-09-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Coinfection Microbiology Discipline Virology Streptococcus Suis Swine Diseases Influenza A Virus, H1n1 Subtype Metabolism Hemagglutinin Glycoproteins, Influenza Virus N-acetylneuraminic Acid Journal Article Virology Influenza A Virus, H3n2 Subtype Animals Swine Streptococcal Infections Protein Binding Orthomyxoviridae Infections Veterinary Trachea
Content Type	Text
Resource Type	Article
Subject	Virology

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