NDLI: Preimplantation processing of ex vivo-derived vascular biomaterials: effects on peripheral cell adhesion.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Uzarski, Joseph S. Van De Walle, Aurore B. McFetridge, Peter S.
Description	Country affiliation: United States Author Affiliation: Uzarski JS ( J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.)
Abstract	The use of ex vivo-derived scaffolds as vascular conduits has shown to be a clinically valid approach to repair or bypass occluded vessels. Implantation of allogeneic tissue grafts requires careful processing to lower immunogenicity and prevent bacterial infection. However, the mechanical/chemical treatments used to prepare biological scaffolds can result in significant alterations to the native structure and surface chemistry, which can affect in vivo performance. Of particular importance for vascular grafts are binding interactions between the implanted biomaterial and host cells from the circulation and adjacent vasculature. Here we present a comparison of four strategies used to decellularize allogeneic human umbilical vein (HUV) scaffolds: ethanol/acetone, sodium chloride, sodium dodecyl sulfate (SDS), or Triton X-100. Scanning electron microscopy revealed that all four techniques achieved removal of native cells from both the lumenal and ablumenal surfaces of HUV grafts. Platelets and promyelocytic HL-60 cells showed preferential binding on the more loosely structured ablumenal surface, although low surface coverage was observed overall by peripheral blood cells. Vascular endothelial cell adhesion was highest on HUV decellularized using ethanol/acetone, and significantly higher than on SDS-processed grafts (p = 0.016). Primary cells showed high viability on the lumenal surface regardless of decellularization technique (over 95% in all cases). These results demonstrate the critical effects of various chemical processing strategies on the adhesive properties of ex vivo-derived vascular grafts. Careful application-specific consideration is warranted when selecting a processing strategy that minimizes innate responses (e.g. thrombosis, inflammation) that are often deleterious to graft survival.
File Format	HTM / HTML
ISSN	15493296
e-ISSN	15524965
DOI	10.1002/jbm.a.34308
Journal	Journal of Biomedical Materials Research Part A
Issue Number	1
Volume Number	101
Language	English
Publisher	Wiley
Publisher Date	2013-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Human Umbilical Vein Endothelial Cells Platelet Adhesiveness Prosthesis Implantation Cell Separation Cell Survival Cytology Pharmacology Blood Vessel Prosthesis Leukocytes Cell Adhesion Drug Effects Hl-60 Cells Discipline Biomedical Engineering Tissue Scaffolds Chemistry Surface Properties Biocompatible Materials
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering

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