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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yi, Shu-min Zhu, Jun-li Fu, Ling-lin Li, Jian-rong |
| Description | Country affiliation: China Author Affiliation: Yi SM ( Food Safety Key Lab of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310035, China.) |
| Abstract | This paper aims to delineate the inhibition mechanism of tea polyphenols (TP) toward Pseudomonas aeruginosa by cell membrane damage. Morphological changes in bacteria treated with TP were investigated by transmission electron microscopy, with results indicating that the primary inhibitory action of TP is to damage bacterial cell membranes. TP also increased the permeability of the outer and inner membranes of P. aeruginosa and disrupted the cell membrane with the release of small cellular molecules. A proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the differences in the membrane proteins of TP-treated P. aeruginosa and those of control samples. Twenty-seven differentially expressed proteins were observed in the treated and the control groups. Most of the proteins identified by MALDI-TOF/TOF MS were enzymes (dihdrollpoamide dehydrogenase 50s ribosomal protein, and so on), which may have induced the metabolic disorder of the bacteria and resulted in their death. |
| File Format | HTM / HTML |
| ISSN | 01681605 |
| Issue Number | 1 |
| Volume Number | 144 |
| e-ISSN | 18793460 |
| Journal | International Journal of Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-11-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell Membrane Microscopy, Electron, Transmission Research Support, Non-u.s. Gov't Discipline Microbiology Plant Extracts Cell Membrane Permeability Pharmacology Pseudomonas Aeruginosa Metabolism Drug Effects Anti-bacterial Agents Journal Article Bacterial Proteins Tea Flavonoids Chemistry Ultrastructure Proteomics Phenols Polyphenols Genetics Discipline Nutritional Sciences Gene Expression Regulation, Bacterial |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Safety, Risk, Reliability and Quality Food Science |
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