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DNA damage and genetic methylation changes caused by Cd in Arabidopsis thaliana seedlings

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Zhaoling Liu, Zhihong Chen, Ruijuan Li, Xiaojun Tai, Peidong Gong, Zongqiang Jia, Chunyun Liu, Wan
Description	Country affiliation: China Author Affiliation: Li Z ( Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China.); Liu Z ( University of Chinese Academy of Sciences, Beijing, China.); Chen R ( Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.); Li X ( Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China.); Tai P ( School of Life and Environmental, Deakin University, Warrnambool, Victoria, Australia.); Gong Z ( Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China.); Jia C ( University of Chinese Academy of Sciences, Beijing, China.); Liu W ( Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China.)
Abstract	Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MASP) techniques are sensitive to deoxyribonucleic acid (DNA) damage and genetic methylation, respectively. Using these 2 techniques, Arabidopsis thaliana cultured with 0 mg/L (control), 0.5 mg/L, 1.5 mg/L, and 5.0 mg/L $Cd^{2+}$ for 16 d was used to analyze the DNA damage and methylation changes as a result of cadmium (Cd). The DNA was amplified by 14 AFLP primer pairs and 13 MSAP primer combinations. In the AFLP experiment, 62 polymorphic sites were found in the patterns of 11 primer combinations and a total of 1116 fragments were obtained in these patterns. There were no polymorphic bands in the remaining 3 pairs. The proportions of polymorphic sites in the 0.5-mg/L $Cd^{2+}$ and 5.0-mg/L $Cd^{2+}$ treatments were significantly different. Seven polymorphic fragments were then separated and successfully sequenced, yielding 6 nucleobase substitutions and 1 nucleobase deletion. Similarly, in the MSAP experiment, the MSAP% and number of demethylated-type bands were unchanged after Cd treatment, but the number of methylated-type bands was increased significantly in the 5.0-mg/L $Cd^{2+}$ treatment group, a finding that may be associated with the AFLP results. The polymorphic bands were also sequenced and the functions of their homologous genes were determined. The DNA damage and methylation changes may be the primary cause of certain pathology changes as a result of Cd uptake in plants. Environ Toxicol Chem 2015;34:2095–2103. © 2015 SETAC
File Format	HTM / HTML
ISSN	07307268
Issue Number	9
Volume Number	34
e-ISSN	15528618
Journal	Environmental Toxicology and Chemistry
Language	English
Publisher	Wiley
Publisher Date	2015-09-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Dna, Plant Toxicity Discipline Environmental Health Molecular Sequence Data Discipline Toxicology Dna Damage Nucleic Acid Amplification Techniques Journal Article Base Sequence Discipline Chemistry Genetics Dna Primers Cadmium Seedling Arabidopsis Metabolism Drug Effects Sequence Analysis, Dna Growth & Development Sequence Alignment Dna Methylation Analysis Mutation Amplified Fragment Length Polymorphism Analysis
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis

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