NDLI: Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides.

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Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides.

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Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bai, Wenhui Zhu, Chao Liu, Jinchuan Yan, Mengmeng Yang, Shuming Chen, Ailiang
Description	Country affiliation: China Author Affiliation: Bai W ( Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.); Zhu C ( Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.); Liu J ( Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.); Yan M ( Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.); Yang S ( Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China.); Chen A ( Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.)
Abstract	Fast immunoassay-based screening methods are unavailable for most small-molecule pesticides because of a lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single-stranded DNA molecules selected through an in vitro process, which can bind to any target including nonimmunogenic small molecules with high affinity and specificity. Although various aptamer-based sensing methods have been developed for antibiotics, microorganisms, heavy metal ions, and biotoxins, there are few reports on aptamer-based methods for quick detection of organophosphorous pesticides. The gold (Au) nanoparticle (AuNP) colorimetric assay is a widely utilized rapid detection method because of properties such as easy operation and visualized results. In the present study, organophosphorous pesticide aptamers were adsorbed on the surface of AuNPs to stabilize the AuNP solution against high concentrations of salt to prevent AuNP aggregation. After the addition of targets, the aptamers binding to the targets are detached from the AuNPs, resulting in aggregation of AuNPs and a color change from red to purple-blue. The proposed method can detect 6 organophosphorous pesticides with good recoveries from 72% to 135% in environmental river water samples. The present study provides a new way for simple, rapid, and multiplex detection of organophosphorous pesticides.
File Format	HTM / HTML
ISSN	07307268
Issue Number	10
Volume Number	34
e-ISSN	15528618
Journal	Environmental Toxicology and Chemistry
Language	English
Publisher	Wiley
Publisher Date	2015-10-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Water Pollutants, Chemical Gold Discipline Environmental Health Pesticides Organophosphorus Compounds Discipline Toxicology Colorimetry Journal Article Chemistry Aptamers, Nucleotide Analysis Discipline Chemistry Fresh Water Circular Dichroism Metal Nanoparticles
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis

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