NDLI: Expression of MCSP and PRAME in conjunctival melanoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Westekemper, Henrike Karimi, Sara Süsskind, Daniela Anastassiou, Gerasimos Freistühler, Michael Meller, Daniel Zeschnigk, Michael Steuhl, Klaus-Peter Bornfeld, Norbert Schmid, Kurt-Werner Grabellus, Florian
Description	Country affiliation: Germany Author Affiliation: Westekemper H ( Department of Ophthalmology, University Hospital of Essen, Hufelandstrasse 55, Essen, Germany. henrike.westekemper@uk-essen.de)
Abstract	BACKGROUND: To analyse the expression of melanoma chondroitin sulfate proteoglycan (MCSP) and the preferentially expressed antigen of melanoma (PRAME) in conjunctival melanoma (CoM), lymph node (LN) metastases of cutaneous melanoma (CM) and conjunctival nevi (CoN) by immunohistology. METHODS: Immunohistology was performed in 70 samples of CoM, 25 of LN metastases of CM and 12 of CoN, and assessed by an immunoreactive score (0-12 points). Statistical analysis was performed to disclose relevant differences in the expression pattern. The diagnostic value of the markers was tested by receiver operating characteristics (ROC) analysis. RESULTS: MCSP and PRAME were expressed at significantly higher levels in CoM and LN metastases of CM than in CoN (p<0.0001). Within CoM, an MCSP expression <9.0 points meant higher risk for recurrences (Cox HR=3.1) and a shorter recurrence-free survival (p=0.002) than an MCSP expression >9.0 points. ROC analysis showed an area under the curve of 91.3% for MCSP (p=0.0002) and 93.8% for PRAME (p<0.0001). CONCLUSIONS: MCSP and PRAME are differentially expressed in conjunctival melanomas and nevi. MCSP might have an impact on the risk for recurrence in being inversely correlated to the event. Both markers have high potential to discriminate CoM from CoN. The results indicate that immunohistological characteristics gain relevance in the assessment of CoM.
File Format	HTM / HTML
ISSN	00071161
Issue Number	10
Volume Number	94
e-ISSN	14682079
Journal	British Journal of Ophthalmology
Language	English
Publisher	BMJ Publishing Group Ltd.
Publisher Date	2010-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunohistochemistry Kaplan-meier Estimate Research Support, Non-u.s. Gov't Secondary Antigens, Neoplasm Mortality Humans Middle Aged Melanoma Neoplasm Recurrence, Local Male Lymphatic Metastasis Metabolism Roc Curve Chondroitin Sulfate Proteoglycans Journal Article Membrane Proteins Nevus Of Ota Discipline Ophthalmology Conjunctival Neoplasms Skin Neoplasms Female Multicenter Study Observer Variation
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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