NDLI: Sequential epiretinal membrane removal with internal limiting membrane peeling in brilliant blue G-assisted macular surgery.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Schumann, Ricarda G. Gandorfer, Arnd Eibl, Kirsten H. Henrich, Paul B. Kampik, Anselm Haritoglou, Christos
Description	Country affiliation: Germany Author Affiliation: Schumann RG ( Department of Ophthalmology, Vitreoretinal and Pathology Unit, Ludwig-Maximilians-University, Mathildenstrasse 8, Munich, Germany. ricarda.schumann@med.uni-muenchen.de)
Abstract	PURPOSE: To assess the selectivity of brilliant blue G (BBG) staining by analysing the morphological components of unstained and stained tissue obtained during epiretinal membrane (ERM) removal with internal limiting membrane (ILM) peeling in BBG-assisted macular surgery. METHODS: Twenty-six surgical specimens were removed from 13 eyes with epiretinal gliosis during vitrectomy using BBG for ERM and ILM peeling. We included eyes with idiopathic macular pucker, idiopathic macular hole and vitreomacular traction syndrome. The dye was injected into the fluid-filled globe. Unstained and stained epiretinal tissue was harvested consecutively and placed into separate containers. All specimens were processed for conventional transmission electron microscopy. RESULTS: The first surgical specimen of all eyes showed no intraoperative staining with BBG and corresponded to masses of cells and collagen. The second surgical specimen demonstrated good staining characteristics and corresponded to the ILM in all patients included. In seven eyes, the ILM specimens were seen with minor cell proliferations such as single cells or a monolayer of cells. Myofibroblasts, fibroblasts and astrocytes were present. In five cases, native vitreous collagen fibrils were found at the ILM. In six of the eyes, ILM specimens were blank. CONCLUSION: Our clinicopathological correlation underlines the selective staining properties of BBG. The residual ILM is selectively stained by BBG even when a small amount of cells and collagen adheres to its vitreal side. To reduce the retinal exposure to the dye, the surgeon might choose to remove the ERM without using the dye, followed by a BBG injection to identify residual ILM.
File Format	HTM / HTML
ISSN	00071161
Issue Number	10
Volume Number	94
e-ISSN	14682079
Journal	British Journal of Ophthalmology
Language	English
Publisher	BMJ Publishing Group Ltd.
Publisher Date	2010-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Retinal Diseases Microscopy, Electron, Transmission Macula Lutea Humans Middle Aged Male Indicators And Reagents Aged, 80 And Over Rosaniline Dyes Journal Article Discipline Ophthalmology Pathology Surgery Female Aged Epiretinal Membrane
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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