NDLI: Experimental microablation of choroidal tissue for autologous retinal pigment epithelium-choroid translocation with the pulsed electron avalanche knife (PEAK-fc).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Treumer, Felix Sattler, Benjamin Bunse, Arnd Klettner, Alexa Hillenkamp, Jost Roider, Johann
Description	Country affiliation: Germany Author Affiliation: Treumer F ( Department of Ophthalmology, University Medical Center Schleswig-Holstein, Arnold-Heller Strasse 3, Kiel, Germany. ftreumer@auge.uni-kiel.de)
Abstract	AIM: To test the microablation of excess graft choroidal tissue with the pulsed electron avalanche knife (PEAK-fc) in an in-vitro model of autologous retinal pigment epithelium (RPE)-choroid translocation. METHODS: Choroidal tissue of porcine RPE-choroid explants was ablated with the PEAK-fc. Tissue morphology was assessed by light microscopy (LM) and scanning electron microscopy (SEM). The amount of ablated choroidal tissue was analysed as a function of three PEAK-fc parameters: (1) amplitude of biphasic voltage (70-100%); (2) distance between choroidal tissue and tip of the PEAK-fc (0-300 µm); and (3) exposure time (2-8 s). RESULTS: LM and SEM showed a smooth plain within the ablation area with well defined cutting edges and preserved adjacent tissue structure. The mean amount of ablated tissue correlated linearly with applied voltage (range 79-120 µm, r=0.34) and distance between choroidal tissue and PEAK-fc tip (range 10-100 µm, r=0.74). The mean amount of ablated tissue increased with exposure time between 2 and 4 s (36-88 µm, r=0.4) and remained constant between 4 and 8 s. CONCLUSION: The PEAK-fc accurately microablates choroidal tissue in-vitro. The adjacent choroidal tissue structure and Bruch's membrane are preserved. Patient studies are required to test the PEAK-fc in RPE-choroid translocation surgery.
File Format	HTM / HTML
ISSN	00071161
Issue Number	10
Volume Number	94
e-ISSN	14682079
Journal	British Journal of Ophthalmology
Language	English
Publisher	BMJ Publishing Group Ltd.
Publisher Date	2010-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Retinal Pigment Epithelium Discipline Ophthalmology Microscopy, Electron, Scanning Animals Swine Ablation Techniques Instrumentation Choroid Transplantation, Autologous Transplantation Journal Article
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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