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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kugimiya, Akimitsu Takamitsu, Emi |
| Description | Author Affiliation: Kugimiya A ( Center for Industry and Public Relations, Hiroshima City University, 3-8-24 Senda-machi, Naka-ku, Hiroshima 730-0052, Japan. Electronic address: kugimiya@hiroshima-cu.ac.jp.) |
| Abstract | An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 µM of histidine and from 15 to 95 µM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0). |
| File Format | HTM / HTML |
| ISSN | 09284931 |
| Issue Number | 8 |
| Volume Number | 33 |
| e-ISSN | 18730191 |
| Journal | Materials Science and Engineering: C |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-12-01 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Subject Keyword | Discipline Materials Science Amino Acyl-trna Synthetases Metabolism Histidine Analysis Lysine Spectrophotometry Histidine-trna Ligase Hydrogen-ion Concentration Lysine-trna Ligase Nad Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering |
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