NDLI: Injectable calcium phosphate-alginate-chitosan microencapsulated MC3T3-E1 cell paste for bone tissue engineering in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Qiao, Pengyan Wang, Juan Xie, Qiufei Li, Fangfang Dong, Limin Xu, Tao
Description	Country affiliation: China Author Affiliation: Qiao P ( Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.)
Abstract	Osteoblasts or stem cells have been delivered into injectable calcium phosphate cement (CPC) to improve its effectiveness and biological function. However, the osteogenic potential of the new construct in vivo has been rarely reported, and there are no reports on alginate-chitosan microencapsulated osteoblasts mixed with CPC. This study aimed to develop alginate-chitosan microencapsulated mouse osteoblast MC3T3-E1 cells (AC-cells), evaluate the osteogenic potential of a calcium phosphate cement complex with these AC-cells (CPC-AC-cell), and trace the implanted MC3T3-E1 cells in vivo. MC3T3-E1 cells were embedded in alginate microcapsules, cultured in osteogenic medium for 7 days, and then covered with chitosan before mixing with a paste of ß-tricalcium phosphate/calcium phosphate cement (ß-TCP/CPC). The construct was injected into the dorsal subcutaneous area of nude mice. Lamellar-bone-like mineralization, newly formed collagen and angiogenesis were observed at 4 weeks. At 8 weeks, areas of newly formed collagen expanded; further absorption of ß-TCP/CPC and osteoid-like structures could be seen. Cell tracing in vivo showed that implanted MC3T3-E1 cells were clearly visible at 2 weeks. These in vivo results indicate that the novel injectable CPC-AC-cell construct is promising for bone tissue engineering applications.
File Format	HTM / HTML
ISSN	09284931
Issue Number	8
Volume Number	33
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2013-12-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Alginates Chemistry Calcium Phosphates Chitosan Tissue Engineering Animals Bone Cements Bone Transplantation Bone And Bones Pathology Capsules Cell Line Glucuronic Acid Hexuronic Acids Mice Mice, Inbred Balb C Mice, Nude Osteoblasts Cytology Osteogenesis Tissue Scaffolds Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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