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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Micallef, Ludovic Battu, Serge Pinon, Aline Cook-Moreau, Jeanne Cardot, Philippe J. P. Delage, Christiane Simon, Alain |
| Description | Country affiliation: France Author Affiliation: Micallef L ( Laboratoire de Chimie Physique, EA 4021 'Biomolécules et Thérapies Anti-tumorales', Université de Limoges, Faculté de Pharmacie, 2 Rue du Dr Marcland, 87025 Limoges Cedex, France.) |
| Abstract | The spontaneously immortalized human keratinocyte cell line HaCaT is widely used as a human keratinocyte model. In a previous comparative study between normal human keratinocytes (NHKs) and HaCaT, we reported that Ca2+ concentrations greater than 1mM induced differentiation in vitro in both cell types, notably characterized by increased expression of differentiation markers keratins 1 (K1), 10 (K10) and involucrin. Surprisingly, cells had a higher proliferative activity than those cultured with low Ca2+ levels. These results raised many questions; in particular concerning the emergence of HaCaT cells subpopulation which would have different differentiation states and/or proliferation rates throughout Ca2+-induced differentiation. To isolate these subpopulations, we used sedimentation field-flow fractionation (SdFFF). Results demonstrated that the most differentiated cells (HC-F1), characterized by the highest expression of keratinocyte differentiation markers, had the lowest proliferative activity. In contrast, less differentiated cells (HC-F2) maintained a higher proliferative activity. SdFFF is a tool to sort differentiated and/or proliferating cells from a total pool previously treated with a Ca2+ concentration inducing differentiation, and can be use to prepare biological models necessary for studying HaCaT cell proliferation after Ca2+-induced differentiation treatment. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Issue Number | 15-16 |
| Volume Number | 878 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Cell Separation Methods Fractionation, Field Flow Keratinocytes Cytology Analysis Of Variance Calcium Pharmacology Cell Differentiation Drug Effects Cell Line, Transformed Cell Proliferation Flow Cytometry Humans Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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