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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Van Der Ham, Maria de Koning, Tom J. Lefeber, Dirk Fleer, André Prinsen, Berthil H. C. M. T. de Sain-van der Velden, Monique G. M. |
| Description | Country affiliation: Netherlands Author Affiliation: van der Ham M ( Department of Metabolic and Endocrine Diseases, University Medical Centre Utrecht, WKZ and Netherlands Metabolomics Centre, The Netherlands.) |
| Abstract | BACKGROUND: Analysis of sialic acid (SA) metabolites in cerebrospinal fluid (CSF) is important for clinical diagnosis. In the present study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) method for free sialic acid (FSA) and total sialic acid (TSA) in human CSF was validated. METHODS: The method utilized a simple sample-preparation procedure of protein precipitation for FSA and acid hydrolysis for TSA. Negative electrospray ionisation was used to monitor the transitions m/z 308.2-->87.0 (SA) and m/z 311.2--> 90.0 ((13)C(3)-SA). Conjugated sialic acid (CSA) was calculated by subtracting FSA from TSA. We established reference intervals for FSA, TSA and CSA in CSF in 217 control subjects. The method has been applied to patients' samples with known differences in SA metabolites like meningitis (n=6), brain tumour (n=2), leukaemia (n=5), and Salla disease (n=1). RESULTS: Limit of detection (LOD) was 0.54 microM for FSA and 0.45 mM for TSA. Intra- and inter-assay variation for FSA (21.8 microM) were 4.8% (n=10) and 10.4% (n=40) respectively. Intra- and inter-assay variation for TSA (35.6 microM) were 9.7% (n=10) and 12.8% (n=40) respectively. Tested patients showed values of TSA above established reference value. CONCLUSION: The validated method allows sensitive and specific measurement of SA metabolites in CSF and can be applied for clinical diagnoses. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Issue Number | 15-16 |
| Volume Number | 878 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods N-acetylneuraminic Acid Cerebrospinal Fluid Tandem Mass Spectrometry Brain Neoplasms Humans Leukemia Meningitis Reproducibility Of Results Sensitivity And Specificity Sialic Acid Storage Disease Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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