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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ni, Xiao-Jia Wang, Zhan-Zhang Shang, De-Wei Zhang, Ming Hu, Jin-Qing Qiu, Chang Wen, Yu-Guan |
| Description | Country affiliation: China Author Affiliation: Ni XJ ( Clinical Laboratory of Phase I, Institution of National Drug Clinical Trials of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Wang ZZ ( Clinical Pharmacy of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Shang DW ( Clinical Laboratory of Phase I, Institution of National Drug Clinical Trials of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Zhang M ( Clinical Laboratory of Phase I, Institution of National Drug Clinical Trials of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Hu JQ ( Clinical Pharmacy of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Qiu C ( Clinical Pharmacy of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China.); Wen YG ( Clinical Laboratory of Phase I, Institution of National Drug Clinical Trials of Guangzhou Brain Hospital, Guangzhou Medical University, 36 MingXin Road, Guangzhou 510370, China. Electronic address: wenyuguandede@163.com.) |
| Abstract | The rapid, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry method (LC-ESI-MS/MS) for the simultaneous estimation and pharmacokinetic investigation of glimepiride and pioglitazone in human plasma has been developed and fully validated. Glimepiride and pioglitazone, compounds which exert synergistic effects on blood glucose control, were investigated in human plasma using deuterium-labeled analogs as internal standards (IS). Liquid-liquid extraction was carried out on 0.2 mL of human plasma using ethyl acetate, and chromatographic separation was performed on an Agilent Eclipse plus C18 column (4.6 mm × 100 mm, 3.5 µm) using a mobile phase consisting of methanol-water-formic acid (95:5:0.1, v/v/v, plus 5mM ammonium acetate) at a flow rate of 0.8 mL/min. To quantify glimepiride, pioglitazone and their IS, multiple reaction monitoring (MRM) transitions of m/z 491.2â352.2, m/z 496.2â357.2, m/z 357.2â134.2 and m/z 361.2â138.2 were performed in positive mode. The total run time was 3.0 min and the elution time was about 2.4 min. The method exhibited good separation of analytes, without interference from endogenous substances. The linear calibration curves were 0.2-250 ng/mL for glimepiride and 0.2-1,250 ng/mL for pioglitazone; the lower limit of quantification (LLOQ) was 0.2 ng/mL for both analytes. Intra- and inter-day reproducibility was less than 10% for glimepiride and less than 5% for pioglitazone, with relative errors ranging from -8.00% to 2.80% at the three concentrations of analytes used for quality control (QC). The matrix effect was negligible and recoveries were similar for each analyte and its IS. Glimepiride and pioglitazone were found to be stable under the assay conditions and the method was successfully applied to the evaluation of pharmacokinetic studies of glimepiride and pioglitazone, following oral doses of 2mg glimepiride tablets and 15 mg pioglitazone tablets to 16 healthy volunteers. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 960 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods Sulfonylurea Compounds Blood Tandem Mass Spectrometry Thiazolidinediones Adult Humans Linear Models Male Reproducibility Of Results Sensitivity And Specificity Chemistry Pharmacokinetics Young Adult Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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