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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Liusheng Lizak, Patricia Dvorak, Christopher C. Aweeka, Francesca Long-Boyle, Janel |
| Description | Author Affiliation: Huang L ( Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, CA 94143, USA. Electronic address: huangl@sfghsom.ucsf.edu.); Lizak P ( Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, CA 94143, USA.); Dvorak CC ( Department of Pediatrics, University of California at San Francisco, San Francisco, USA.); Aweeka F ( Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, CA 94143, USA.); Long-Boyle J ( Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, CA 94143, USA.) |
| Abstract | A method for quantification of fludarabine (FDB) and clofarabine (CFB) in human plasma was developed with an API5000 LC-MS/MS system. FDB and CFB were extracted from EDTA plasma samples by protein precipitation with trichloroacetic acid. Briefly, 50 µL plasma sample was mixed with 25 µL internal standard (50 ng/mL aqueous 2-Cl-adensosine) and 25 µL 20% trichloroacetic acid, centrifuged at 25,000 × g (20,000 rpm) for 3 min, and then transfered to an autosampler vial. The extracted sample was injected onto an Eclipse extend C18 column (2.1 mm×150 mm, 5 µm) and eluted with 1mM NH4OH (pH 9.6) - acetonitrile in a gradient mode. Electrospray ionization in positive mode (ESI(+)) and multiple reaction monitoring (MRM) were used, and ion pairs 286/134 for FDB, 304/170 for CFB and 302/134 for the internal standard were selected for quantification. The retention times were typically 3.72 min for FDB, 4.34 min for the internal standard, 4.79 min for CFB. Total run time was 10 min per sample. Calibration range was 0.5-80 ng/mL for CFB and 2-800 ng/mL for FDB. The method was applied to a clinical pharmacokinetic study in pediatric patients. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| e-ISSN | 1873376X |
| DOI | 10.1016/j.jchromb.2014.04.045 |
| Journal | Journal of Chromatography B |
| Volume Number | 960 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Discipline Analytical Chemistry Adenine Nucleotides Blood Arabinonucleosides Chromatography, Liquid Tandem Mass Spectrometry Vidarabine Analogs & Derivatives Chemistry Pharmacokinetics Linear Models Reproducibility Of Results Sensitivity And Specificity Spectrometry, Mass, Electrospray Ionization Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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