NDLI: Simultaneous determination of dextromethorphan, dextrorphan and doxylamine in human plasma by HPLC coupled to electrospray ionization tandem mass spectrometry: application to a pharmacokinetic study.

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Simultaneous determination of dextromethorphan, dextrorphan and doxylamine in human plasma by HPLC coupled to electrospray ionization tandem mass spectrometry: application to a pharmacokinetic study.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Donato, J. L. Koizumi, F. Pereira, A. S. Mendes, G. D. De Nucci, G.
Description	Country affiliation: Brazil Author Affiliation: Donato JL ( ATCGen Biotechnology, Campinas, São Paulo, Brazil. donato@atcgen.com.br)
Abstract	In the present study, a fast, sensitive and robust method to quantify dextromethorphan, dextrorphan and doxylamine in human plasma using deuterated internal standards (IS) is described. The analytes and the IS were extracted from plasma by a liquid-liquid extraction (LLE) using diethyl-ether/hexane (80/20, v/v). Extracted samples were analyzed by high performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Chromatographic separation was performed by pumping the mobile phase (acetonitrile/water/formic acid (90/9/1, v/v/v) during 4.0min at a flow-rate of 1.5 mL minâ»¹ into a Phenomenex Gemini® C18, 5 µm analytical column (150 × 4.6 mm i.d.). The calibration curve was linear over the range from 0.2 to 200 ng mLâ»¹ for dextromethorphan and doxylamine and 0.05 to 10 ng mLâ»¹ for dextrorphan. The intra-batch precision and accuracy (%CV) of the method ranged from 2.5 to 9.5%, and 88.9 to 105.1%, respectively. Method inter-batch precision (%CV) and accuracy ranged from 6.7 to 10.3%, and 92.2 to 107.1%, respectively. The run-time was for 4 min. The analytical procedure herein described was used to assess the pharmacokinetics of dextromethorphan, dextrorphan and doxylamine in healthy volunteers after a single oral dose of a formulation containing 30 mg of dextromethorphan hydrobromide and 12.5mg of doxylamine succinate. The method has high sensitivity, specificity and allows high throughput analysis required for a pharmacokinetic study.
File Format	HTM / HTML
ISSN	15700232
Volume Number	899
e-ISSN	1873376X
Journal	Journal of Chromatography B
Language	English
Publisher	Elsevier
Publisher Date	2012-06-15
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods Dextromethorphan Blood Dextrorphan Doxylamine Spectrometry, Mass, Electrospray Ionization Adolescent Adult Pharmacokinetics Female Humans Male Middle Aged Sensitivity And Specificity Tandem Mass Spectrometry Young Adult Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry

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