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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Watterson, J. H. Imfeld, A. B. Cornthwaite, H. C. |
| Description | Author Affiliation: Watterson JH ( Department of Forensic Science, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON, Canada P3E 2C6. Electronic address: jwatterson@laurentian.ca.); Imfeld AB ( Department of Forensic Science, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON, Canada P3E 2C6.); Cornthwaite HC ( Department of Forensic Science, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON, Canada P3E 2C6.) |
| Abstract | Microwave assisted extraction (MAE) followed by microplate solid phase extraction (MPSPE) coupled with ultra high performance liquid chromatography (UHPLC) for the semi-quantitative determination of colchicine, 3-demethyl colchicine and 2-demethyl colchicine in postmortem rat bone is described. Rats (n=4) received 50mg/kg colchicine (i.p), and euthanized by CO2 asphyxiation. Remains decomposed to skeleton outdoors and vertebral bones were collected cleaned, and ground to a fine powder. Powdered bone underwent MAE using methanol in a closed microwave system, followed by MPSPE and analysis using UHPLC-PDA. MAE analyte stability was assessed and found to be stable for at least 60 min irradiation time. The majority (>95%) of each analyte was recovered after 15 min. The MPSPE-UHPLC method was linear between 10 and 2,000 ng/mL, with coefficients of variation <20% in triplicate analysis, with a limit of detection of 10 ng/mL for each of the three analytes. Following MAE for 30 min (80°C, 1200W), MPSPE-UHPLC analysis of vertebral bone of colchicine-exposed rats detected colchicine (1.8-4.1 µg/g), 3-demethyl colchicine (0.77-1.8 µg/g) and 2-demethyl colchicine (0.43-0.80 µg/g) in all samples assayed. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 960 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Bone And Bones Chemistry Chromatography, High Pressure Liquid Methods Colchicine Analogs & Derivatives Analysis Solid Phase Extraction Animals Pharmacokinetics Microwaves Rats Rats, Wistar Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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