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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tompsett, A. R. Park, J. W. Zhang, X. Jones, P. D. Newsted, J. L. Au, D. W. T. Chen, E. X. H. Yu, R. Wu, R. S. S. Kong, R. Y. C. Giesy, J. P. Hecker, M. |
| Description | Country affiliation: United States Author Affiliation: Tompsett AR ( Department of Zoology, Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA. amber.tompsett@usask.ca) |
| Abstract | A whole-animal tissue section in situ hybridization (ISH) system with radio-labeled probes was developed to detect differential gene expression among tissues of the small, oviparous teleost fish, Japanese medaka (Oryzias latipes). Because of its tissue- and gender-specific expression, gonadal aromatase (CYP19a) was selected as a model gene to demonstrate the potential of the system. The ISH system was validated with a 7d exposure to the model aromatase inhibitor, fadrozole. Fadrozole did not affect the magnitude of gene expression in testes, but significantly up-regulated CYP19a gene expression in ovaries. These results were confirmed with quantitative real-time-polymerase chain reaction (RT-PCR). Histological evaluation revealed that females exposed to 100microg/L fadrozole lacked mature oocytes. Male gonadal morphology was normal in all treatments. The ISH method developed in this study allowed tissue-specific resolution of gene expression in a whole animal model, as well as the ability to analyze cellular morphological detail in the same organism. |
| File Format | HTM / HTML |
| ISSN | 01476513 |
| Issue Number | 4 |
| Volume Number | 72 |
| e-ISSN | 10902414 |
| Journal | Ecotoxicology and Environmental Safety |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Environmental Sciences Gene Expression Physiology Oryzias Animals Autoradiography Body Weight Drug Effects Dna Primers Dna, Complementary Biosynthesis Genetics Estrogen Antagonists Pharmacology Fadrozole Female Fresh Water Analysis Growth Image Processing, Computer-assisted Immunohistochemistry In Situ Hybridization Male Ovary Metabolism Pathology Rna Probes Rna, Messenger Reverse Transcriptase Polymerase Chain Reaction Testis Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pollution Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health |
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