NDLI: Proteomic analysis shows the upregulation of erythrocyte dematin in zinc-restricted human subjects.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ryu, Moon-Suhn Guthrie, Gregory J. Maki, Alyssa B. Aydemir, Tolunay B. Cousins, Robert J.
Description	Country affiliation: United States Author Affiliation: Ryu MS ( Food Science and Human Nutrition Department, Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL, USA.)
Abstract	BACKGROUND: Although the importance of adequate zinc intake has been known for decades, the estimated global prevalence of zinc deficiency remains high. This substantiates the need for a specific and sensitive status assessment tool. OBJECTIVE: The objective was to evaluate erythrocyte zinc transporters as candidate molecules with the potential of being a biomarker of dietary zinc status in humans. DESIGN: A 24-d observational study with acclimation (7 d, 10.4 mg Zn/d), zinc-depletion (10 d, 0.3 mg Zn/d), and zinc-repletion (7 d, 29.5 mg Zn/d) phases was conducted in healthy men (n = 9). Proteomic approaches including Western blot analyses and tandem mass spectrometry were implemented to identify the zinc responsiveness of selected red blood cell membrane proteins. RESULTS: Zinc transporter 1 (ZnT1) and Zrt/Irt-like proteins ZIP8 and ZIP10 were detected in human erythrocyte membranes. No effects of short-term dietary zinc depletion were observed on the amounts of these proteins. However, changes in a cytoskeletal protein, dematin, by zinc depletion were identified through the nonspecific signals produced by an anti-ZIP8 antibody. This response was further validated by a dematin-specific antibody and with erythrocytes collected from mice fed a zinc-deficient diet. CONCLUSIONS: The presence of ZnT1, ZIP8, and ZIP10 in human red blood cells implicates their role in the regulation of cellular zinc metabolism in the human erythroid system. The zinc responsiveness of membrane dematin suggests its capability to serve as a biomarker for dietary zinc depletion and its involvement in impaired erythroid membrane fragility by zinc restriction. This trial was registered at clinicaltrials.gov as NCT01221129.
File Format	HTM / HTML
ISSN	00029165
e-ISSN	19383207
DOI	10.3945/ajcn.111.032862
Journal	American Journal of Clinical Nutrition
Issue Number	5
Volume Number	95
Language	English
Publisher	American Society for Nutrition
Publisher Date	2012-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Nutritional Sciences Erythrocytes Metabolism Microfilament Proteins Proteome Zinc Administration & Dosage Deficiency Amino Acid Sequence Animals Biological Markers Blood Blotting, Western Carrier Proteins Cation Transport Proteins Mice Mice, Inbred C57bl Molecular Sequence Data Nutritional Status Tandem Mass Spectrometry Up-regulation Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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