NDLI: Vaccination with the repeat β-hCG C-terminal peptide carried by heat shock protein-65 (HSP65) for inducing antitumor effects

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Jie Zhang, Yankai Wang, Huaqian Gao, Zhenqiu Wang, Zeyu Liu, Bin Zhang, Xiuhua Du, Mingzhu Huang, Xiao Xu, Maolei Wu, Jie Li, Taiming Liu, Jingjing Cao, Rongyue
Description	Author Affiliation: Yang J ( Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing, 210009, People's Republic of China.)
Abstract	The β-subunit of human chorionic gonadotropin (β-hCG) is ectopically expressed in various types of cancer and has been utilized as an antigenic target in anti-cancer vaccines. In view of the low immunogenicity of this self-peptide, we designed a method based on the isocaudamer technique to generate 14 tandem repeats of the 10-residue sequence X of β-hCG (109-118). These tandemly repeated copies were then combined with β-hCG C-terminal 37 peptides (CTP37) and finally fused to mycobacterial heat-shock protein 65 (HSP65) to construct a fusion protein HSP65-X14-βhCGCTP37 as an immunogen. In this study, BALB/c female mice were immunized via subcutaneous injection of the designed protein. Humoral immune and cellular immune responses were effectively elicited. A high titer of anti-β-hCG antibody was detected in immunized mice sera by enzyme-linked immunosorbent assay and verified by Western blot analysis. The fusion protein, HSP65-X14-β-hCGCTP37, effectively inhibited the growth of Ehrlich ascites carcinoma in mice. These results suggest that HSP65-X14-βhCGCTP37 may be an effective tumor vaccine, and the use of multiple tandem repeats of a certain epitope is an effective method to overcome the low immunogenicity of self-peptide antigens.
File Format	HTM / HTML
ISSN	10104283
Issue Number	5
Volume Number	33
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2012-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Bacterial Proteins Genetics Cancer Vaccines Immunology Chaperonin 60 Chorionic Gonadotropin, Beta Subunit, Human Peptides Recombinant Fusion Proteins Animals Antibodies Blood Base Sequence Cell Line, Tumor Female Gene Order Humans Lymphocyte Activation Lymphocytes Mice Neoplasms Therapy Vaccination Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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