NDLI: Effects of SASH1 on lung cancer cell proliferation, apoptosis, and invasion in vitro.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, En-guo Chen, Yanfan Dong, Liang-liang Zhang, Ji-song
Description	Country affiliation: China Author Affiliation: Chen EG ( Department of Respiratory Medicine, Sir Run Run Shaw Hospital of Zhejiang University, No. 3, East Qingchun Rd, Hangzhou, 310016, China. chenenguo@126.com)
Abstract	The purposes of this study were to investigate the effects of the SASH1 gene on the growth, proliferation, apoptosis, invasiveness, and metastatic potential of lung cancer cells and explore the potential use of SASH1 for the treatment of human lung cancer. The SASH1 gene was cloned into the pcDNA3.1 eukaryotic expression vector, and SASH1 shRNA were designed and constructed. The resulting constructs were transfected into A549 human lung cancer cells, and the changes in the relevant biological characteristics of the cells overexpressing SASH1 and cells with downregulated expression of SASH1 were analyzed using the MTT assay, transwell invasion assay, and flow cytometry. The effects of the SASH1 gene on the expression of cyclin D1, Bcl-2, and MMP-2/9 were also concurrently examined. In the A549 cells from the pcDNA3.1-SASH1 transfected group, cell viability, proliferation, and migration were significantly reduced compared to the control cells (p = 0.039, p = 0.013), and a cell cycle arrest in G1 was observed. The A549 cells transfected with the SASH1 shRNA demonstrated significantly higher cell viabilities, proliferation, and migration compared to the control cells (p = 0.012, p = 0.045). Additionally, the percentage of A549 cells undergoing apoptosis was significantly higher in the pcDNA3.1-SASH1 transfected cells and significantly lower in the SASH1 shRNA transfected cells compared to the control cells (p = 0.010, p = 0.000). The cyclin D1, Bcl-2, and MMP-9/2 protein expression levels were significantly lower in the pcDNA3.1-SASH1-transfected cells and were significantly higher in the SASH1 shRNA-transfected cells than that in the control cells. The SASH1 gene may inhibit A549 cell growth and proliferation as well as promote cellular apoptosis. The overexpression of the SASH1 gene may also be related to the decreased migration of A549 human lung cancer cells.
File Format	HTM / HTML
ISSN	10104283
Issue Number	5
Volume Number	33
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2012-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Apoptosis Genetics Cell Movement Lung Neoplasms Pathology Tumor Suppressor Proteins Cell Cycle Cell Line, Tumor Cell Proliferation Cyclin D1 Metabolism Gene Expression Humans Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Proto-oncogene Proteins C-bcl-2 Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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