NDLI: Curcumin: a unique antioxidant offers a multimechanistic approach for management of hepatocellular carcinoma in rat model.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ahmed, Hanaa H. Shousha, Wafaa Gh Shalby, Aziza B. El-Mezayen, Hatem A. Ismaiel, Nora N. Mahmoud, Nadia S.
Description	Author Affiliation: Ahmed HH ( Hormones Department, Medical Research Division, National Research Centre, 12411, Dokki, Giza, Egypt, hanaaomr@yahoo.com.)
Abstract	This study was designed to investigate the role of curcumin against hepatocellular carcinoma (HCC) induced in rats. Forty rats were divided into five groups. Group (1) was negative control. Groups (2), (4), and (5) were orally administrated N-nitrosodiethylamine for HCC induction, then group (2) was left untreated, and group (4) was treated orally with curcumin, while group (5) was intraperitoneally injected with doxorubicin. Group (3) was served as curcumin control group. Serum alpha-fetoprotein, alpha L-fucosidase and vascular endothelial growth factor levels were analyzed. Gamma glutamyl transferase (GGT) and heat shock protein gp96 (HSPgp96) gene expressions were detected by RT-PCR. The immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) and Ki-67 expressions was performed. Apoptosis was detected using DNA fragmentation assay. Also, histological investigation of liver tissue was achieved. Untreated HCC group showed significant elevation in the studied biochemical markers and significant upregulation in GGT and HSPgp96 gene expression as well as marked increase in PCNA and Ki-67 expression. Furthermore, this group revealed no DNA fragmentation. Histological investigation of liver tissue sections in HCC group revealed a typical anaplasia. On the other hand, the curcumin-treated group showed a significant depletion in the studied tumor markers and a significant downregulation in GGT and HSPgp96 gene expression. Also, this group displayed remarkable decrease in PCNA and Ki-67 expression. Moreover, this group revealed an obvious DNA fragmentation. Interestingly, treatment with curcumin showed remarkable improvement in the histological features of liver tissue. This study revealed the promising therapeutic role of curcumin against hepatocellular carcinoma owing to its antiangiogenic, antiproliferative, and apoptotic effects.
File Format	HTM / HTML
ISSN	10104283
Issue Number	3
Volume Number	36
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2015-03-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Antioxidants Pharmacology Carcinoma, Hepatocellular Drug Therapy Curcumin Liver Neoplasms Angiogenesis Inhibitors Animals Apoptosis Drug Effects Tumor Markers, Biological Metabolism Cell Proliferation Dna Fragmentation Down-regulation Heat-shock Proteins Ki-67 Antigen Liver Male Proliferating Cell Nuclear Antigen Rats Rats, Wistar Up-regulation Alpha-fetoproteins Gamma-glutamyltransferase Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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