NDLI: Cellular immunoisolation for islet transplantation by a novel dual porosity electrospun membrane.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Krishnan, L. Clayton, L. R. Boland, E. D. Reed, R. M. Hoying, J. B. Williams, S. K.
Description	Country affiliation: United States Author Affiliation: Krishnan L ( Cardiovascular Innovation Insititute, Louisville, Kentucky 40202, USA.)
Abstract	Immunoisolation strategies have the potential to impact the treatment of several diseases, such as hemophilia, Parkinson's and endocrine disorders, such as parathryroid disorders and diabetes. The hallmark of these disease states is the amelioration of the disease process by replacement of the deficient protein. Naturally, several cellular therapeutic strategies like genetically modified host cells, stem cells, donor cells, or even complex tissues like pancreatic islets have been investigated. Current evidence suggests that successful strategies must incorporate considerations for local hypoxia, vascularity, and immunoisolation. Additional regulatory concerns also include safe localization of implanted therapeutic cells to allow for monitoring, dose adjustment, or removal when indicated. Local hypoxia and cellular toxicity can be detrimental to the survival of freshly implanted pancreatic islets, leading to a need for a larger initial number of islets or repeated implantation procedures. The lack of adequate donors and the large number of islet equivalents needed to achieve euglycemic states amplify the nature of this problem. We have developed a novel immunoisolation device based on electrospun nylon, primarily for islet transplantation, such that the inner component functions as a cellular barrier while allowing diffusion, whereas the outer component can be optimized for tissue integration and accelerated vascularization. Devices explanted after subcutaneous implantation in wild-type B6 mice after a period of 30 days show vascular elements in the outer layer of the electrospun device. The inner layer when intact functioned as an effective barrier to cellular infiltration. The preimplantation of such a device, with a relatively thin inner barrier membrane, will allow for adequate vascularization and reduce postimplantation hypoxia. This study demonstrates the feasibility of an electrospun isolation device that can be easily assembled, modified by varying the electrospinning parameters, and functionalized with surface-active molecules to accelerate vascularization.
File Format	HTM / HTML
ISSN	00411345
e-ISSN	18732623
DOI	10.1016/j.transproceed.2011.10.031
Journal	Transplantation Proceedings
Issue Number	9
Volume Number	43
Language	English
Publisher	Elsevier
Publisher Date	2011-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Transplantation__semicolon__medicine Cell Separation Islets Of Langerhans Transplantation Animals Cell Culture Techniques Instrumentation Cell-derived Microparticles Anoxia Immunohistochemistry Insulin Metabolism Membranes, Artificial Mice Microscopy, Electron, Scanning Porosity Transplantation, Heterologous Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Transplantation Surgery

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Islet Preparation Purity Is Overestimated, and Less Pure Fractions Have Lower Post-Culture Viability Before Clinical Allotransplantation

Morphological changes of post-isolation of caprine pancreatic islet.

Human islet viability and function is maintained during high-density shipment in silicone rubber membrane vessels.

Culture of impure human islet fractions in the presence of alpha-1 antitrypsin prevents insulin cleavage and improves islet recovery.

Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.

Anodic aluminium oxide membranes for immunoisolation with sufficient oxygen supply for pancreatic islets

Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets.

Glial cell line-derived neurotrophic factor enhances human islet posttransplantation survival.

New alginate microcapsule system for angiogenic protein delivery and immunoisolation of islets for transplantation in the rat omentum pouch.

Cellular immunoisolation for islet transplantation by a novel dual porosity electrospun membrane.

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Islet Preparation Purity Is Overestimated, and Less Pure Fractions Have Lower Post-Culture Viability Before Clinical Allotransplantation

Morphological changes of post-isolation of caprine pancreatic islet.

Human islet viability and function is maintained during high-density shipment in silicone rubber membrane vessels.

Culture of impure human islet fractions in the presence of alpha-1 antitrypsin prevents insulin cleavage and improves islet recovery.

Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.

Anodic aluminium oxide membranes for immunoisolation with sufficient oxygen supply for pancreatic islets

Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets.

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