| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Du, Kangli Luo, Zhimin Guo, Pengqi Tang, Weili Wu, Ningli Zheng, Penglei Du, Wei Zeng, Aiguo Jing, Wanghui Chang, Chun Fu, Qiang |
| Description |
Country affiliation: China Author Affiliation: Du K ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Luo Z ( Department of Pharmacy, Tianjin Huanhu Hospital, Tianjin, China.); Guo P ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Tang W ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Wu N ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Zheng P ( Department of Pharmacy, Hospital of Stomatology Attached Xi'an Jiaotong University, Xi'an, China.); Du W ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Zeng A ( Department of Pharmacy, Xi'an First Hospital, Xi'an, China.); Jing W ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Chang C ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.); Fu Q ( School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.) |
| Abstract |
Highly selective molecularly imprinted polymers on the surface of silica gels were prepared by a sol-gel process and used as solid-phase extraction adsorbents for the specific recognition, enrichment and detection of cloxacilloic acid in cloxacillin. The obtained polymers were characterized by scanning electron microscopy, FTIR spectroscopy, nitrogen adsorption and desorption, elemental analysis and thermogravimetric analysis. The imprinted polymers not only possessed high adsorption capacity (6.5 µg/mg), but also exhibited fast adsorption kinetics (they adsorb 80% of the maximum amount within 20 min) and excellent selectivity (the imprinted factor was 3.6). A method using the imprinted polymers as solid-phase extraction adsorbents coupled with high-performance liquid chromatography was established with good specificity, linearity (r = 0.9962), precision (ranging from 0.5 to 6.7%), accuracy (ranging from 93.9 to 97.7%) and extraction recoveries (ranging from 78.8 to 89.8%). The limits of detection and quantification were 0.07 and 0.25 mg/g, respectively. This work could provide a promising method in the enrichment, extraction and detection of allergenic impurities in the manufacture, storage and application of cloxacillin. |
| File Format |
HTM / HTML |
| ISSN |
16159306 |
| Issue Number |
3 |
| Volume Number |
39 |
| e-ISSN |
16159314 |
| Journal |
Journal of Separation Science |
| Language |
English |
| Publisher |
Wiley-VCH |
| Publisher Date |
2016-02-01 |
| Publisher Place |
Germany |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Discipline Chemistry Cloxacillin Chemistry Molecular Imprinting Solid Phase Extraction Adsorption Evaluation Studies As Topic Humans Microscopy, Electron, Scanning Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Analytical Chemistry Filtration and Separation |
