NDLI: Evaluation and optimization of the metal-binding properties of a complex ligand for immobilized metal affinity chromatography

Please wait, while we are loading the content...

Screening anaphylactic components of MaiLuoNing injection by using rat basophilic leukemia-2H3 cell membrane chromatography coupled with HPLC-ESI-TOF-MS.

Extraction of quinolones from milk samples using bentonite/magnetite nanoparticles before determination by high-performance liquid chromatography with fluorimetric detection.

Preparation of molecularly imprinted polymeric fibers using a single bifunctional monomer for the solid-phase microextraction of parabens from environmental solid samples.

Preparation and evaluation of a molecularly imprinted sol-gel material as the solid-phase extraction adsorbents for the specific recognition of cloxacilloic acid in cloxacillin.

Determination of polycyclic aromatic hydrocarbons in soy isoflavone nutraceutical products by gas chromatography coupled to triple quadrupole tandem mass spectrometry.

Rapid determination of flavonoids in licorice and comparison of three licorice species.

Combinative application of pH-zone-refining and conventional high-speed counter-current chromatography for preparative separation of caged polyprenylated xanthones from gamboge.

Multiple monolithic fiber solid-phase microextraction based on a polymeric ionic liquid with high-performance liquid chromatography for the determination of steroid sex hormones in water and urine.

Reversed-phase ion-pair high-performance liquid chromatography assay of polyprenyl diphosphate oligomer homologues.

Isolation of a furan fatty acid from Hevea brasiliensis latex employing the combined use of pH-zone-refining and conventional countercurrent chromatography.

Enhanced resolution of Mentha piperita volatile fraction using a novel medium-polarity ionic liquid gas chromatography stationary phase.

Microwave-assisted extraction and high-throughput monolithic-polymer-based micro-solid-phase extraction of organophosphorus, triazole, and organochlorine residues in apple.

Improvement of mineral oil saturated and aromatic hydrocarbons determination in edible oil by liquid-liquid-gas chromatography with dual detection.

Characterization of the multiple components of Acanthopanax Senticosus stem by ultra high performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry.

Evaluation and optimization of the metal-binding properties of a complex ligand for immobilized metal affinity chromatography

Identification and evaluation of the chemical similarity of Yindan xinnaotong samples by ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry fingerprinting.

Preparation of novel alkaline pH-responsive copolymers for the formation of recyclable aqueous two-phase systems and their application in the extraction of lincomycin.

Computer-aided design and synthesis of magnetic molecularly imprinted polymers with high selectivity for the removal of phenol from water.

Rapid extraction and determination of 25 bioactive constituents in Alpinia oxyphylla using microwave extraction with ultra high performance liquid chromatography with tandem mass spectrometry.

Ultra high performance liquid chromatography with mass spectrometry for the rapid analysis and global characterization of multiple constituents from Zibu Piyin Recipe.

Year: 2016 Volume: 39 Issue: 2

Year: 2016 Volume: 39 Issue: 1

Year: 2015 Volume: 38

Year: 2014 Volume: 37

Year: 2013 Volume: 36

Year: 2012 Volume: 35

Year: 2011 Volume: 34

Year: 2010 Volume: 33

Year: 2009 Volume: 32

Year: 2008 Volume: 31

Year: 2007 Volume: 30

Year: 2005 Volume: 28

Evaluation and optimization of the metal-binding properties of a complex ligand for immobilized metal affinity chromatography

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Bin Li, Rong Li, Shiyu Chen, Xiaoli Yang, Kaidi Chen, Guoliang Ma, Xiaoxun
Description	Author Affiliation: Chen B ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.); Li R ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.); Li S ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.); Chen X ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.); Yang K ( School of Natural and Applied Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi Province, P. R. China.); Chen G ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.); Ma X ( Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi Province, P. R. China.)
Abstract	The simultaneous determination of two binding parameters for metal ions on an immobilized metal affinity chromatography column was performed by frontal chromatography. In this study, the binding parameters of $Cu^{2+}$ to l-glutamic acid were measured, the metal ion-binding characteristics of the complex ligand were evaluated. The linear correlation coefficients were all greater than 99%, and the relative standard deviations of two binding parameters were 0.58 and 0.059%, respectively. The experiments proved that the frontal chromatography method was accurate, reproducible, and could be used to determine the metal-binding parameters of the affinity column. The effects of buffer pH, type, and concentration on binding parameters were explored by uniform design experiment. Regression, matching and residual analyses of the models were performed. Meanwhile, the optimum-binding conditions of $Cu^{2+}$ on the l-glutamic acid-silica column were obtained. Under these binding conditions, observations and regression values of two parameters were similar, and the observation values were the best. The results demonstrated that high intensity metal affinity column could be effectively prepared by measuring and evaluating binding parameters using frontal chromatography combined with a uniform design experiment. The present work provided a new mode for evaluating and preparing immobilized metal affinity column with good metal-binding behaviors.
File Format	HTM / HTML
ISSN	16159306
Issue Number	3
Journal	Journal of Separation Science
Volume Number	39
e-ISSN	16159314
Language	English
Publisher	Wiley-VCH
Publisher Date	2016-02-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Filtration and Separation

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Affinity purification of metalloprotease from marine bacterium using immobilized metal affinity chromatography.

Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins.

Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides.

New α-amino phenylalanine tetrazole ligand for immobilized metal affinity chromatography of proteins

Novel bis(5-methyltetrazolium)amine ligand-bonded stationary phase with reduced leakage of metal ions in immobilized metal affinity chromatography of proteins

Optimization of antibody immobilization for on-line or off-line immunoaffinity chromatography.

Study of pepsin phosphorylation using immobilized metal affinity chromatography

Purification of Glutathione by Immobilized Metal Ion Affinity Chromatography with Thiourea as the Ligand

Chromatographic separation simulation of metal-chelating peptides from surface plasmon resonance binding parameters

Evaluation and optimization of the metal-binding properties of a complex ligand for immobilized metal affinity chromatography

Similar Documents

Affinity purification of metalloprotease from marine bacterium using immobilized metal affinity chromatography.

Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins.

Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides.

New α-amino phenylalanine tetrazole ligand for immobilized metal affinity chromatography of proteins

Novel bis(5-methyltetrazolium)amine ligand-bonded stationary phase with reduced leakage of metal ions in immobilized metal affinity chromatography of proteins

Optimization of antibody immobilization for on-line or off-line immunoaffinity chromatography.

Study of pepsin phosphorylation using immobilized metal affinity chromatography

Purification of Glutathione by Immobilized Metal Ion Affinity Chromatography with Thiourea as the Ligand

Chromatographic separation simulation of metal-chelating peptides from surface plasmon resonance binding parameters

Evaluation and optimization of the metal-binding properties of a complex ligand for immobilized metal affinity chromatography