| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Castellani, Laura Perry, Christopher G. R. Macpherson, Rebecca E. K. Root-McCaig, Jared Huber, Jason S. Arkell, Alicia M. Simpson, Jeremy A. Wright, David C. |
| Description |
Author Affiliation: Castellani L ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Perry CG ( Faculty of Health, School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada.); Macpherson RE ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Root-McCaig J ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Huber JS ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Arkell AM ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Simpson JA ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada); Wright DC ( Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada) |
| Abstract |
The purpose of this investigation was to determine whether exercise-induced increases in adipose tissue interleukin 6 (IL-6) signaling occurred as part of a larger proinflammatory response to exercise and whether the induction of IL-6 signaling with acute exercise was altered in trained mice in parallel with changes in the IL-6 receptor complex. Sedentary and trained C57BL/6J mice were challenged with an acute bout of exercise. Adipose tissue and plasma were collected immediately and 4 h afterward and analyzed for changes in indices of IL-6 signaling, circulating IL-6, markers of adipose tissue inflammation, and expression/content of IL-6 receptor and glycoprotein 130 (gp130). In untrained mice, IL-6 mRNA increased immediately after exercise, and increases in indices of IL-6 signaling were increased 4 h after exercise in epididymal, but not inguinal adipose tissue. This occurred independent of increases in plasma IL-6 and alterations in markers of inflammation. When compared with untrained mice, in trained mice, acute exercise induced the expression of gp130 and IL-6 receptor alpha (IL-6R ), and training increased the protein content of these. Acute exercise induced the expression, and training increased the protein content, of glycoprotein 130 and IL-6R and was associated with a more rapid increase in markers of IL-6 signaling in epididymal adipose tissue from trained compared with untrained mice. The ability of exogenous IL-6 to increase phosphorylation of STAT3 was similar between groups. Our findings demonstrate that acute exercise increases IL-6 signaling in a depot-dependent manner, likely through an autocrine/paracrine mechanism. This response is initiated more rapidly after exercise in trained mice, potentially as a result of increases in IL-6R and gp130. |
| File Format |
HTM / HTML |
| ISSN |
87507587 |
| e-ISSN |
15221601 |
| Journal |
Journal of Applied Physiology |
| Issue Number |
11 |
| Volume Number |
119 |
| Language |
English |
| Publisher |
American Physiological Society |
| Publisher Date |
2015-12-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Physiology Adipose Tissue Physiopathology Inflammation Interleukin-6 Physical Conditioning, Animal Physical Exertion Signal Transduction Metabolism Animals Cytokine Receptor Gp130 Epididymis Fatty Acids, Nonesterified Blood Mice Mice, Inbred C57bl Phosphorylation Receptors, Interleukin-6 Stat3 Transcription Factor Sedentary Lifestyle Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Physiology Physiology (medical) Sports Science |
