| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Percival, Michael E. Martin, Brian J. Gillen, Jenna B. Skelly, Lauren E. MacInnis, Martin J. Green, Alex E. Tarnopolsky, Mark A. Gibala, Martin J. |
| Description |
Author Affiliation: Percival ME ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada); Martin BJ ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada); Gillen JB ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada); Skelly LE ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada); MacInnis MJ ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada); Green AE ( Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada.); Tarnopolsky MA ( Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada.); Gibala MJ ( Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada) |
| Abstract |
We tested the hypothesis that ingestion of sodium bicarbonate (NaHCO3) prior to an acute session of high-intensity interval training (HIIT) would augment signaling cascades and gene expression linked to mitochondrial biogenesis in human skeletal muscle. On two occasions separated by â¼1 wk, nine men (mean ± SD: age 22 ± 2 yr, weight 78 ± 13 kg, VÌO(2 peak) 48 ± 8 ml·kg(-1)·min(-1)) performed 10 × 60-s cycling efforts at an intensity eliciting â¼90% of maximal heart rate (263 ± 40 W), interspersed with 60 s of recovery. In a double-blind, crossover manner, subjects ingested a total of 0.4 g/kg body weight NaHCO3 before exercise (BICARB) or an equimolar amount of a placebo, sodium chloride (PLAC). Venous blood bicarbonate and pH were elevated at all time points after ingestion (P < 0.05) in BICARB vs. PLAC. During exercise, muscle glycogen utilization (126 ± 47 vs. 53 ± 38 mmol/kg dry weight, P < 0.05) and blood lactate accumulation (12.8 ± 2.6 vs. 10.5 ± 2.8 mmol/liter, P < 0.05) were greater in BICARB vs. PLAC. The acute exercise-induced increase in the phosphorylation of acetyl-CoA carboxylase, a downstream marker of AMP-activated protein kinase activity, and p38 mitogen-activated protein kinase were similar between treatments (P > 0.05). However, the increase in PGC-1 mRNA expression after 3 h of recovery was higher in BICARB vs. PLAC (approximately sevenfold vs. fivefold compared with rest, P < 0.05). We conclude that NaHCO3 before HIIT alters the mRNA expression of this key regulatory protein associated with mitochondrial biogenesis. The elevated PGC-1 mRNA response provides a putative mechanism to explain the enhanced mitochondrial adaptation observed after chronic HIIT supplemented with NaHCO3 in rats. |
| File Format |
HTM / HTML |
| ISSN |
87507587 |
| e-ISSN |
15221601 |
| Journal |
Journal of Applied Physiology |
| Issue Number |
11 |
| Volume Number |
119 |
| Language |
English |
| Publisher |
American Physiological Society |
| Publisher Date |
2015-12-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Physiology Physiology Rna, Messenger Biosynthesis Sodium Bicarbonate Pharmacology Transcription Factors Bicarbonates Blood Cross-over Studies Double-blind Method Glycogen Metabolism Heart Rate Mitochondria, Muscle Muscle, Skeletal Phosphorylation P38 Mitogen-activated Protein Kinases Genetics Randomized Controlled Trial Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Physiology Physiology (medical) Sports Science |
