NDLI: Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bartnikas, Lisa M. Gurish, Michael F. Burton, Oliver T. Leisten, Sabine Janssen, Erin Oettgen, Hans C. Beaupré, Jacqueline Lewis, Christopher N. Austen, K. Frank Schulte, Stephanie Hornick, Jason L. Geha, Raif S. Oyoshi, Michiko K.
Description	Country affiliation: United States Author Affiliation: Bartnikas LM ( Division of Immunology, Boston Children's Hospital, Boston, Mass 02115, USA.)
Abstract	BACKGROUND: Sensitization to food antigen can occur through cutaneous exposure. OBJECTIVE: We sought to test the hypothesis that epicutaneous sensitization with food antigen predisposes to IgE-mediated anaphylaxis on oral allergen challenge. METHODS: BALB/c mice were epicutaneously sensitized by repeated application of ovalbumin (OVA) to tape-stripped skin over 7 weeks or orally immunized with OVA and cholera toxin (CT) weekly for 8 weeks and then orally challenged with OVA. Body temperature was monitored, and serum mouse mast cell protease 1 levels were determined after challenge. Tissue mast cell (MC) counts were examined by using chloroacetate esterase staining. Levels of serum OVA-specific IgE and IgG(1) antibodies and cytokines in supernatants of OVA-stimulated splenocytes were measured by means of ELISA. Serum IL-4 levels were measured by using an in vivo cytokine capture assay. RESULTS: Epicutaneously sensitized mice exhibited expansion of connective tissue MCs in the jejunum, increased serum IL-4 levels, and systemic anaphylaxis after oral challenge, as evidenced by decreased body temperature and increased serum mouse mast cell protease 1 levels. Intestinal MC expansion and anaphylaxis were IgE dependent because they did not occur in epicutaneously sensitized IgE(-/-) mice. Mice orally immunized with OVA plus CT did not have increased serum IL-4 levels, expanded intestinal MCs, or anaphylaxis after oral challenge, despite OVA-specific IgE levels and splenocyte cytokine production in response to OVA stimulation, which were comparable with those of epicutaneously sensitized mice. CONCLUSION: Epicutaneously sensitized mice, but not mice orally immunized with antigen plus CT, have expansion of intestinal MCs and IgE-mediated anaphylaxis after single oral antigen challenge. IgE is necessary but not sufficient for food anaphylaxis, and MC expansion in the gut can play an important role in the development of anaphylaxis.
File Format	HTM / HTML
ISSN	00916749
e-ISSN	10976825
DOI	10.1016/j.jaci.2012.11.032
Journal	Journal of Allergy and Clinical Immunology
Issue Number	2
Volume Number	131
Language	English
Publisher	Elsevier
Publisher Date	2013-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Immunology Anaphylaxis Immunology Food Hypersensitivity Immunoglobulin E Jejunum Mast Cells Skin Administration, Cutaneous Allergens Animals Antibodies Antigens Body Temperature Chemokine Ccl2 Cholera Toxin Immunoglobulin G Interleukin-4 Mice Mice, Inbred Balb C Ovalbumin Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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