NDLI: Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oka, Tatsuya Rios, Eon J. Tsai, Mindy Kalesnikoff, Janet Galli, Stephen J.
Description	Author Affiliation: Oka T ( Department of Pathology, Stanford University School of Medicine, Stanford, Calif.)
Abstract	BACKGROUND: Rapid desensitization transiently prevents severe allergic reactions, allowing administration of life-saving therapies in previously sensitized patients. However, the mechanisms underlying successful rapid desensitization are not fully understood. OBJECTIVES: We sought to investigate whether the mast cell (MC) is an important target of rapid desensitization in mice sensitized to exhibit IgE-dependent passive systemic anaphylaxis in vivo and to investigate the antigen specificity and underlying mechanisms of rapid desensitization in our mouse model. METHODS: C57BL/6 mice (in vivo) or primary isolated C57BL/6 mouse peritoneal mast cells (PMCs; in vitro) were passively sensitized with antigen-specific anti-2,4-dinitrophenyl IgE, anti-ovalbumin IgE, or both. MCs were exposed over a short period of time to increasing amounts of antigen (2,4-dinitrophenyl-human serum albumin or ovalbumin) in the presence of extracellular calcium in vitro or by means of intravenous administration to sensitized mice in vivo before challenging the mice with or exposing the PMCs to optimal amounts of specific or irrelevant antigen. RESULTS: Rapidly exposing mice or PMCs to progressively increasing amounts of specific antigen inhibited the development of antigen-induced hypothermia in sensitized mice in vivo and inhibited antigen-induced PMC degranulation and prostaglandin D2 synthesis in vitro. Such MC hyporesponsiveness was induced antigen-specifically and was associated with a significant reduction in antigen-specific IgE levels on MC surfaces. CONCLUSIONS: Rapidly exposing MCs to progressively increasing amounts of antigen can both enhance the internalization of antigen-specific IgE on the MC surface and also desensitize these cells in an antigen-specific manner in vivo and in vitro.
File Format	HTM / HTML
ISSN	00916749
e-ISSN	10976825
DOI	10.1016/j.jaci.2013.05.004
Journal	Journal of Allergy and Clinical Immunology
Issue Number	4
Volume Number	132
Language	English
Publisher	Elsevier
Publisher Date	2013-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Immunology Anaphylaxis Therapy Antigens Immunology Desensitization, Immunologic Immunoglobulin E Mast Cells 2,4-dinitrophenol Animals Antibodies, Anti-idiotypic Antibody Specificity Administration & Dosage Metabolism Hypersensitivity Mice Mice, Inbred C57bl Ovalbumin Time Factors Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Rapid polyclonal desensitization with antibodies to IgE and FcÎµRI .

Tolerance induction with T cell-dependent protein antigens induces regulatory sialylated IgGs.

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.

Oral immunotherapy induces IgG antibodies that act through FcÎ³RIIb to suppress IgE-mediated hypersensitivity.

Mechanisms of allergen-specific desensitization.

Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.

Rapid desensitization of mice with anti-FcÎ³RIIb/FcÎ³RIII mAb safely prevents IgG-mediated anaphylaxis.

Antigen induces chronic idiotype suppression.

IgE antibodies, FcÎµRI , and IgE-mediated local anaphylaxis can limit snake venom toxicity.

Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.

Similar Documents

Rapid polyclonal desensitization with antibodies to IgE and FcÎµRI .

Tolerance induction with T cell-dependent protein antigens induces regulatory sialylated IgGs.

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.

Oral immunotherapy induces IgG antibodies that act through FcÎ³RIIb to suppress IgE-mediated hypersensitivity.

Mechanisms of allergen-specific desensitization.

Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.

Rapid desensitization of mice with anti-FcÎ³RIIb/FcÎ³RIII mAb safely prevents IgG-mediated anaphylaxis.

Antigen induces chronic idiotype suppression.

IgE antibodies, FcÎµRI , and IgE-mediated local anaphylaxis can limit snake venom toxicity.

Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.