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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fareez, Ismail M. Lim, Siong Meng Mishra, Rakesh K. Ramasamy, Kalavathy |
| Description | Country affiliation: Malaysia Author Affiliation: Fareez IM ( Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia); Lim SM ( Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia); Mishra RK ( Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia); Ramasamy K ( Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia) |
| Abstract | The vulnerability of probiotics at low pH and high temperature has limited their optimal use as nutraceuticals. This study addressed these issues by adopting a physicochemical driven approach of incorporating Lactobacillus plantarum LAB12 into chitosan (Ch) coated alginate-xanthan gum (Alg-XG) beads. Characterisation of Alg-XG-Ch, which elicited little effect on bead size and polydispersity, demonstrated good miscibility with improved bead surface smoothness and L. plantarum LAB12 entrapment when compared to Alg, Alg-Ch and Alg-XG. Sequential incubation of Alg-XG-Ch in simulated gastric juice and intestinal fluid yielded high survival rate of L. plantarum LAB12 (95%) at pH 1.8 which in turn facilitated sufficient release of probiotics (>7 log CFU/g) at pH 6.8 in both time- and pH-dependent manner. Whilst minimising viability loss at 75 and 90 °C, Alg-XG-Ch improved storage durability of L. plantarum LAB12 at 4 °C. The present results implied the possible use of L. plantarum LAB12 incorporated in Alg-XG-Ch as new functional food ingredient with health claims. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 72 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Adaptation, Physiological Drug Effects Alginates Pharmacology Chitosan Lactobacillus Plantarum Physiology Microspheres Polysaccharides, Bacterial Temperature Calorimetry, Differential Scanning Cells, Immobilized Metabolism Freezing Glucuronic Acid Hexuronic Acids Hot Temperature Hydrogen-ion Concentration Ultrastructure Microbial Viability Spectroscopy, Fourier Transform Infrared X-ray Diffraction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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