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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, Jie Xu, Li-Li Zhou, Qin-Wei Hao, Shu-Xian Zhou, Tao Xie, Hu-Jun |
| Description | Author Affiliation: Xu J ( School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.); Xu LL ( School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.); Zhou QW ( School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.); Hao SX ( Department of Food processing and Safety Control, South China Sea Fishery Research Institute, Guangzhou, PR China.); Zhou T ( School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: taozhou@zjgsu.edu.cn.); Xie HJ ( Department of Applied Chemistry, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: hjxie@zjgsu.edu.cn.) |
| Abstract | In the present study, purification and characterization of enzymatic hydrolysates of polysaccharide from Enteromorpha prolifera (HPE) are described. HPE was sequentially purified by DEAE Cellulose-52 chromatography and Sephadex G-100 chromatography to afford three fractions, namely, PHPE1, PHPE2, and PHPE3. Molecular weights of these three fractions were measured to be 103, 45.4, and 9.8kDa, respectively, using high performance gel permeation chromatography (HPGPC). The three fractions were evaluated for their antioxidant activities by determining their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radicals. PHPE2 was found to possess the strongest scavenging ability. GC-MS analysis indicates that PHPE2 is mainly composed of mannose, xylose, and glucose. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 81 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Antioxidants Isolation & Purification Pharmacology Polysaccharides Ulva Chemistry Biphenyl Compounds Chromatography, Gel Chromatography, Ion Exchange Free Radical Scavengers Hydrolysis Hydroxyl Radical Molecular Weight Picrates Spectrophotometry, Ultraviolet Superoxides Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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