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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kazem-Farzandi, Najmeh Taheri-Kafrani, Asghar Haertlé, Thomas |
| Description | Country affiliation: Iran Author Affiliation: Kazem-Farzandi N ( Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 81746-73441, Iran.); Taheri-Kafrani A ( Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 81746-73441, Iran. Electronic address: a.taheri@ast.ui.ac.ir.); Haertlé T ( UR 1268, INRA BIA-FIPL, B.P. 71627, 44316 Nantes Cedex 03, France.) |
| Abstract | ß-Lactoglobulin (ß-LG) is a member of lipocalin superfamily of transporters for small hydrophobic molecules. ß-LG is also one of the major allergens in milk. Despite a lot of researches on decreasing of cow's milk allergenicity, the effects of the mutation of ß-LG on its recognition by IgE from cow's milk allergy (CMA) patients have not been investigated. We described here the expression in the yeast Pichia pastoris of a mutant ß-LG, in which Alanine 86 was changed into Glutamine (Ala86Gln; a mutation on one of the major epitopes of the protein). The purity and native like folded structure of the recombinant Ala86Gln have been demonstrated using circular dichroism, HPLC, SDS-PAGE and mass spectrometry. The effect of the mutation on the binding of IgE from CMA patients to mutant protein was evaluated by ELISA methods and the results showed that the mutation of Ala-86 was associated with weaker binding of IgE from CMA patients to Ala86Gln mutant protein. Subsequently, the binding of various ligands such as retinol, palmitic acid, resveratrol and serotonin, with native, recombinant wild type and Ala86Gln mutant ß-LGs were investigated by fluorescence spectroscopy and an improvement on the binding affinity of the mutated protein to various ligands was observed. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 81 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Amino Acid Substitution Codon Lactoglobulins Genetics Metabolism Ligands Mutation Animals Cattle Chromatography, High Pressure Liquid Circular Dichroism Immunoglobulin E Blood Immunology Chemistry Isolation & Purification Milk Adverse Effects Milk Hypersensitivity Protein Binding Recombinant Proteins Spectrometry, Mass, Electrospray Ionization Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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